<Original>Seasonal Fluctuation on the Proteins in the Bark of Sophora japonica L. Analyzed by Enzyme Immuno-Assay.

Abstract

Electrophoretical analysis showed that four proteins were existed in winter and disappeared in summer in the bark of Sophora japonica. The two major proteins of them were purified and the antibodies were raised against them. The seasonal changes of them were measured by enzyme immuno-assay, and the profiles of them were compared. The two profiles were quite different, especially in autumn. One increased linearly from summer to winter, whereas the other increased with fluctuation during the same period. These results suggested that these proteins may have the distinct roles in the bark.