<Original>Dehydrogenative Polymerization of Monolignols by Peroxidase and H_2O_2 in a Dialysis Tube. I. : Preparation of Highly Polymerized DHPs

Abstract

Coniferyl alcohol gave a highly polymerized DHP whose molecular weight was higher than that of bamboo MWL by dialysis membrane method. The DHPs obtained by this method were almost insoluble in any solvents but swelled in DMSO, DMF, formamide and pyridine. The molecular weight of soluble fraction of the DHPs showed to be higher than that of the DHP prepared by "Zutropf" method on gel nitration chromatography with Sephadex LH-60 but smaller than that of bamboo MWL with Sephadex G-200. However, thermal softening point (Ts) (175℃) of the total DHP obtained by dialysis membrane method was higher than that of bamboo MWL (160℃). Thermal softening points of DHPs were shifted to the higher temperature with increase of peroxidase concentration in the dialysis tube. The results seem to indicate that a concentrated enzyme solution is required to prepare the highly polymerized DHP.