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タイトル: | Association of a 66 kDa homolog of Chlamydomonas DC2, a subunit of the outer arm docking complex, with outer arm dynein of sperm flagella in the ascidian Ciona intestinalis |
著者: | Ushimaru, Yuji Konno, Aru Kaizu, Maiko Ogawa, Kazuo Satoh, Nori Inaba, Kazuo |
著者名の別形: | 佐藤, 矩行 |
キーワード: | dynein flagella sperm motility axoneme ascidian |
発行日: | Aug-2006 |
出版者: | Zoological Society of Japan |
誌名: | Zoological Science |
巻: | 23 |
号: | 8 |
開始ページ: | 679 |
終了ページ: | 687 |
抄録: | We previously identified a 66 kDa axonernal protein (Ci-Axp66.0) in sperm of the ascidian Ciona intestinalis. Here we found that Ci-Axp66.0 shows sequence similarity to the DC2 subunit of the Chiamydomonas outer arm docking complex. Analysis of secondary structure of Ci-Axp66.0 suggested that the N-terminal two-thirds of the molecule is rich in coiled coil structure, as in Chiamydomonas DC2. Immunogold localization revealed that it is located in the vicinity of outer arm dynein. Ci-Axp66.0 was partly extracted from the axonemes by a high salt solution and co-purified with outer arm dynein. This co-purification was not affected by the absence of Mg2l in isolation buffer, indicating that Ci-Axp66.0 is associated with outer arm dynein. These results suggest that Ci-Axp66.0 is a component of the outer arm dynein docking complex in the axonemes of Ciona sperm. |
著作権等: | (c) 日本動物学会 / Zoological Society of Japan |
URI: | http://hdl.handle.net/2433/57190 |
DOI(出版社版): | 10.2108/zsj.23.679 |
出現コレクション: | Zoological Science |
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