ダウンロード数: 369
| タイトル: | Fluorescent in situ hybridization to ascidian chromosomes |
| 著者: | Shoguchi, Eiichi Ikuta, Tetsuro Yoshizaki, Fumiko Satou, Yutaka    https://orcid.org/0000-0001-5193-0708 (unconfirmed)Satoh, Nori Asano, Katsutoshi Saiga, Hidetoshi Nishikata, Takahito |
| 著者名の別形: | 佐藤, 矩行 佐藤, ゆたか |
| キーワード: | ascidians fluorescent in situ hybridization mapping of genes |
| 発行日: | Feb-2004 |
| 出版者: | Zoological Society of Japan |
| 誌名: | Zoological Science |
| 巻: | 21 |
| 号: | 2 |
| 開始ページ: | 153 |
| 終了ページ: | 157 |
| 抄録: | The draft genome of the ascidian Ciona intestinalis has been sequenced. Mapping of the genome sequence to the Ciona 14 haploid chromosomes is essential for future studies of the genome-wide control of gene expression in this basal chordate. Here we describe an efficient protocol for fluorescent in situ hybridization for mapping genes to the Ciona chromosomes. We demonstrate how the locations of two BAC clones can be mapped relative to each other. We also show that this method is efficient for coupling two so-far independent scaffolds into one longer scaffold when two BAC clones represent sequences located at either end of the two scaffolds. |
| 著作権等: | (c) 日本動物学会 / Zoological Society of Japan |
| URI: | http://hdl.handle.net/2433/57201 |
| DOI(出版社版): | 10.2108/zsj.21.153 |
| 出現コレクション: | Zoological Science |
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