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dc.contributor.author | Esaki, Nobuyoshi | en |
dc.contributor.author | Yoshimura, Tohru | en |
dc.contributor.author | Soda, Kenji | en |
dc.contributor.author | Lim, Young Hee | en |
dc.date.accessioned | 2008-08-25T06:07:17Z | - |
dc.date.available | 2008-08-25T06:07:17Z | - |
dc.date.issued | 1999-03 | - |
dc.identifier.issn | 1342-0321 | - |
dc.identifier.uri | http://hdl.handle.net/2433/65185 | - |
dc.description.abstract | Pyridoxal 5'-phosphate-dependent amino acid racemases of broad substrate specificity catalyze transamination as a side-reaction. We studied the stereospecificities for hydrogen abstraction from C-4' of the bound pyridoxamine 5'-phosphate during transamination from pyridoxamine 5'-phosphate to pyruvate catalyzed by three amino acid racemases of broad substrate specificity. When the enzymes were incubated with (4'S)- or (4'R)-[4'-3H]- pyridoxamine 5'-phosphate in the presence of pyruvate, tritium was released into the solvent from both pyridoxamine 5'-phosphates. Thus, these enzymes abstract a hydrogen non-stereospecifically from C-4' of the coenzyme in contrast to the other pyridoxal 5'-phosphate-dependent enzymes so far studied which catalyze the stereospecific hydrogen removal. Amino acid racemase of broad substrate specificity from Pseudomonas putida produced D- and L-glutamate from a-ketoglutarate through the transamination with L-ornithine. Because glutamate does not serve as a substrate for racemization, the enzyme catalyzed the non-stereospecific overall transamination between L-ornithine and a-ketoglutarate. The cleavage and formation of the C-H bond at C-4' of the coenzyme and C-2 of the substrate thus occurs non-stereospecifically on both sides of the plane of the coenzyme-substrate complex intermediate. Amino acid racemase of broad substrate specificity is the first example of a pyridoxal enzyme catalyzing non-stereospecific transamination. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Institute for Chemical Research, Kyoto University | en |
dc.subject | Amino acid racemase | en |
dc.subject | Stereochemistry | en |
dc.subject | Pyridoxal phosphate | en |
dc.subject.ndc | 430 | - |
dc.title | Non-stereospecific Transamination Catalyzed by Pyridoxal Phosphate-dependent Amino Acid Racemases of Broad Substrate Specificity (MOLECULAR BIOFUNCTION-Molecular Microbial Science) | en |
dc.type | article | - |
dc.type.niitype | Article | - |
dc.identifier.ncid | AA11061308 | - |
dc.identifier.jtitle | ICR Annual Report | en |
dc.identifier.volume | 5 | - |
dc.identifier.spage | 46 | - |
dc.identifier.epage | 47 | - |
dc.textversion | publisher | - |
dc.sortkey | 25 | - |
dcterms.accessRights | open access | - |
dc.identifier.pissn | 1342-0321 | - |
出現コレクション: | Vol.5 (1998) |
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