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タイトル: | Structural Basis for Reaction Mechanism and Drug Delivery System of Chromoprotein Antitumor Antibiotic C-1027 (BIOORGANIC CHEMISTRY-Bioactive Chemistry) |
著者: | Okuno, Yasushi Sugiura, Yukio |
キーワード: | Antitumor antibiotic Enediyne Drug delivery system NMR structure DNA cleavage |
発行日: | Mar-2000 |
出版者: | Institute for Chemical Research, Kyoto University |
誌名: | ICR Annual Report |
巻: | 6 |
開始ページ: | 40 |
終了ページ: | 41 |
抄録: | Antitumor antibiotic C-1027 that is regarded as a natural model of drug delivery system, consists of a carrier apoprotein (Apo) and an enediyne chromophore (Chr). We have compared three solution structures of the DNA-Chr complex, Apo-Chr complex, and free Chr determined by high-resolution NMR experiments. The guest molecule, C-1027 chromophore, showed two distinct binding modes fitted to binding sites of the hosts (target DNA and carrier Apo). The novel Chr interacts with DNA through its benzoxazolinate and aminosugar moieties, and also with Apo through the benzoxazolinate and macrocyclic moieties. The superposition of Chrs in these three states clearly revealed conformational deviation of the 16-membered macrocyclic moiety containing intra-chlorophenol ring. Ab initio calculations supported good correlation between the reactivity and the conformational alteration of Chr induced in hosts. The present results provide molecular basis and implication for the host-recognition mode, the reaction mechanism, and drug delivery system of chromoprotein C-1027. |
URI: | http://hdl.handle.net/2433/65229 |
出現コレクション: | Vol.6 (1999) |
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