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i0289-0003-19-11-1257.pdf | 1.13 MB | Adobe PDF | 見る/開く |
タイトル: | Cultivation and characterization of planarian neuronal cells isolated by fluorescence activated cell sorting (FACS) |
著者: | Asami, Maki Nakatsuka, Tsuyoshi Hayashi, Tetsutaro Kou, Kenji Kagawa, Hiroaki Agata, Kiyokazu |
著者名の別形: | 阿形, 清和 |
キーワード: | planarian neuron FACS extracellular matrix plasticity |
発行日: | Nov-2002 |
出版者: | Zoological Society of Japan |
誌名: | Zoological Science |
巻: | 19 |
号: | 11 |
開始ページ: | 1257 |
終了ページ: | 1265 |
抄録: | Studies using molecular markers have revealed that planarians possess a highly organized brain. Here we separated brain neurons from dissociated planarian head cells by fluorescence activated cell sorting (FACS), and characterized them by single cell PCR analysis and cell culture. Dissociated cells were labeled with three different fluorescent dyes, Hoechst 33258, Merocyanine 540, and Propidium Iodide (PI), and fractioned by FACS. Interestingly, we have succeeded in identifying a cell fraction specific to the head, which we have named the head-abundant cell fraction (HAC). Most of the HAC expressed neuron-specific genes and proteins. When they were cultured in vitro, they showed an ability to extend neurites on several types of extracellular matrices (ECMs), and, depending on the ECM type used, presented a high level of plasticity in morphology and gene expression. |
著作権等: | (c) 日本動物学会 / Zoological Society of Japan |
URI: | http://hdl.handle.net/2433/85305 |
DOI(出版社版): | 10.2108/zsj.19.1257 |
出現コレクション: | Zoological Science |
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