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タイトル: | The Dugesia ryukyuensis Database as a Molecular Resource for Studying Switching of the Reproductive System |
著者: | Ishizuka, Hideyuki Maezawa, Takanobu Kawauchi, Junpei Nodono, Hanae Hirao, Yukako Nishimura, Osamu Nakagawa, Haruka Sekii, Kiyono Tasaka, Kenta Tarui, Hiroshi Agata, Kiyokazu Hoshi, Motonori Kobayashi, Kazuya Sakakibara, Yasubumi Matsumoto, Midori |
著者名の別形: | 阿形, 清和 |
キーワード: | planarian EST analysis sexualization asexual-sexual switch gene ontology |
発行日: | Jan-2007 |
出版者: | Zoological Society of Japan |
誌名: | Zoological Science |
巻: | 24 |
号: | 1 |
開始ページ: | 31 |
終了ページ: | 37 |
抄録: | The planarian Dugesia ryukyuensis reproduces both asexually and sexually, and can switch from one mode of reproduction to the other. We recently developed a method for experimentally switching reproduction of the planarian from the asexual to the sexual mode. We constructed a cDNA library from sexualized D. ryukyuensis and sequenced and analyzed 8,988 expressed sequence tags (ESTs). The ESTs were analyzed and grouped into 3,077 non-redundant sequences, leaving 1,929 singletons that formed the basis of unigene sets. Fifty-six percent of the cDNAs analyzed shared similarity (E-value<1E -20) with sequences deposited in NCBI. Highly redundant sequences encoded granulin and actin, which are expressed in the whole body, and other redundant sequences encoded a Vasa-like protein, which is known to be a component of germ-line cells and is expressed in the ovary, and Y-protein, which is expressed in the testis. The sexualized planarian expressed sequence tag database (http://planaria.bio.keio.ac.jp/planaria/) is an open-access, online resource providing access to sequence, classification, clustering, and annotation data. This database should constitute a powerful tool for analyzing sexualization in planarians. |
著作権等: | (c) 日本動物学会 / Zoological Society of Japan |
URI: | http://hdl.handle.net/2433/85330 |
DOI(出版社版): | 10.2108/zsj.24.31 |
出現コレクション: | Zoological Science |
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