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j.bbrc.2009.08.136.pdf | 2.06 MB | Adobe PDF | 見る/開く |
タイトル: | MicroRNA-133 regulates the expression of GLUT4 by targeting KLF15 and is involved in metabolic control in cardiac myocytes |
著者: | Horie, Takahiro Ono, Koh https://orcid.org/0000-0002-4163-980X (unconfirmed) Nishi, Hitoo Iwanaga, Yoshitaka Nagao, Kazuya Kinoshita, Minako Kuwabara, Yasuhide Takanabe, Rieko Hasegawa, Koji Kita, Toru Kimura, Takeshi |
著者名の別形: | 尾野, 亘 |
キーワード: | Cardiomyocyte GLUT4 MicroRNA |
発行日: | Nov-2009 |
出版者: | Elsevier |
誌名: | Biochemical and Biophysical Research Communications |
巻: | 389 |
号: | 2 |
開始ページ: | 315 |
終了ページ: | 320 |
抄録: | GLUT4 shows decreased levels in failing human adult hearts. We speculated that GLUT4 expression in cardiac muscle may be fine-tuned by microRNAs. Forced expression of miR-133 decreased GLUT4 expression and reduced insulin-mediated glucose uptake in cardiomyocytes. A computational miRNA target prediction algorithm showed that KLF15 is one of the targets of miR-133. It was confirmed that over-expression of miR-133 reduced the protein level of KLF15, which reduced the level of the downstream target GLUT4. Cardiac myocytes infected with lenti-decoy, in which the 3′UTR with tandem sequences complementary to miR-133 was linked to the luciferase reporter gene, had decreased miR-133 levels and increased levels of GLUT4. The expression levels of KLF15 and GLUT4 were decreased at the left ventricular hypertrophy and congestive heart failure stage in a rat model. The present results indicated that miR-133 regulates the expression of GLUT4 by targeting KLF15 and is involved in metabolic control in cardiomyocytes. |
著作権等: | c 2009 Elsevier Inc. All rights reserved. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/86176 |
DOI(出版社版): | 10.1016/j.bbrc.2009.08.136 |
PubMed ID: | 19720047 |
出現コレクション: | 学術雑誌掲載論文等 |
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