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DCフィールド | 値 | 言語 |
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dc.contributor.author | Yano, Yoshiaki | en |
dc.contributor.author | Matsuzaki, Katsumi | en |
dc.contributor.alternative | 松崎, 勝巳 | ja |
dc.contributor.alternative | 矢野, 義明 | ja |
dc.date.accessioned | 2009-10-28T07:50:36Z | - |
dc.date.available | 2009-10-28T07:50:36Z | - |
dc.date.issued | 2009-10 | - |
dc.identifier.issn | 00052736 | - |
dc.identifier.uri | http://hdl.handle.net/2433/86184 | - |
dc.description.abstract | Instead of using reconstituted proteoliposomes, in situ investigations of membrane proteins in living cell membranes are important because the heterogeneous and dynamic nature of biomembranes significantly affects their behavior. Protein-specific labeling is a key technique for the detection of a target protein by fluorescence measurements, particularly fluorescence microscopy. However, conventional genetic fusion with fluorescent proteins has several shortcomings. Post-translational labeling methods using a genetically encodable tag and synthetic probes targeting to the tag can overcome these limitations. This review summarizes emerging tag–probe techniques for labeling specific membrane proteins and their applications, including endocytotic internalization, partitioning to specific membrane domains, interprotein interactions, and conformational changes. | en |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | c 2009 Elsevier B.V. All rights reserved. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Coiled-coil | en |
dc.subject | Fluorescence imaging | en |
dc.subject | Membrane protein | en |
dc.subject | Tag-probe labeling | en |
dc.title | Tag-probe labeling methods for live-cell imaging of membrane proteins | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00564635 | - |
dc.identifier.jtitle | Biochimica et biophysica acta | en |
dc.identifier.volume | 1788 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 2124 | - |
dc.identifier.epage | 2131 | - |
dc.relation.doi | 10.1016/j.bbamem.2009.07.017 | - |
dc.textversion | author | - |
dc.identifier.pmid | 19646952 | - |
dcterms.accessRights | open access | - |
dc.identifier.pissn | 0006-3002 | - |
出現コレクション: | 学術雑誌掲載論文等 |
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