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タイトル: | Surface modification of islets with PEG-lipid for improvement of graft survival in intraportal transplantation. |
著者: | Teramura, Yuji Iwata, Hiroo |
著者名の別形: | 寺村, 裕治 |
発行日: | 15-Sep-2009 |
出版者: | Lippincott, Williams & Wilkins |
誌名: | Transplantation |
巻: | 88 |
号: | 5 |
開始ページ: | 624 |
終了ページ: | 630 |
抄録: | BACKGROUND: Transplantation of islets of Langerhans (islets) is a promising technique for treating insulin-dependent diabetes mellitus (type I). One unsolved issue is the early graft loss due to inflammatory reactions triggered by blood coagulation and complement activation that occurs immediately after transplantation into the liver through the portal vein. Several proposed approaches for improvement of the graft survival include heparin coating and covalent poly(ethylene glycol) (PEG) conjugation. We previously have studied the improvement of graft survival by modification of islet surfaces using amphiphilic PEG-conjugated phospholipid and bioactive molecules. Here, we analyzed the effect of PEG-modification on the improvement of graft survival immediately after intraportal transplantation into streptozotocin-induced diabetic mice. METHODS: The surface of hamster islets was modified with PEG-lipid. PEG-lipid modified islets (PEG-islets) were transplanted into the liver through the portal vein of streptozotocin-induced diabetic mice. We measured the graft survival periods and blood insulin levels immediately after intraportal transplantation to determine the cell damage to islets. Histocytochemical analyses of liver were also performed postintraportal transplantation. RESULTS: The graft survival of PEG-islets was significantly prolonged compared with bare islets in livers of diabetic mice. Reduction of blood insulin level within 60 min after transplantation of PEG-islets suggests that the cell damage observed immediately after transplantation could be suppressed by surface modification with PEG in comparison with bare islets. CONCLUSION: Our approach for the improvement of graft survival will be useful in the clinical setting. |
著作権等: | c 2009 Lippincott Williams & Wilkins, Inc. 許諾条件により本文は2010-10-01に公開。 |
URI: | http://hdl.handle.net/2433/87308 |
DOI(出版社版): | 10.1097/TP.0b013e3181b230ac |
PubMed ID: | 19741458 |
出現コレクション: | 学術雑誌掲載論文等 |
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