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dc.contributor.author | Niidome, Tetsuhiro | en |
dc.contributor.author | Nonaka, Hideki | en |
dc.contributor.author | Akaike, Akinori | en |
dc.contributor.author | Kihara, Takeshi | en |
dc.contributor.author | Sugimoto, Hachiro | en |
dc.contributor.alternative | 新留, 徹広 | ja |
dc.date.accessioned | 2009-12-18T02:28:32Z | - |
dc.date.available | 2009-12-18T02:28:32Z | - |
dc.date.issued | 2009-12-18 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/2433/88960 | - |
dc.description.abstract | We recently demonstrated that microglia as multipotential stem cells give rise to microtubule-associated protein 2 (MAP2)-positive and glial fibrillary acidic protein (GFAP)-positive cells and that microglia-derived MAP2-positive cells possess properties of functional neurons. In this study, we investigated the role of fibroblast growth factor (FGF) signaling in the molecular mechanism underlying the generation of microglia-derived MAP2-positive and GFAP-positive cells. Real-time quantitative PCR analyses demonstrated that mRNA levels of a family of three FGF receptors, Fgfr1-3, were upregulated in microglia treated with 70% fetal bovine serum (FBS). Immunocytochemical analyses demonstrated that basic FGF (bFGF) promoted the generation of microglia-derived MAP2-positive and GFAP-positive cells, and the FGF receptor tyrosine kinase inhibitor SU5402 and the MEK inhibitor PD98059 both inhibited this process. Western blot analyses demonstrated that bFGF increased phosphorylated ERK1/2 levels without altering total ERK1/2 levels. These results suggest that bFGF promotes the generation of microglia-derived MAP2-positive and GFAP-positive cells via FGF receptors and the ERK-MAP kinase pathway. | en |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | c 2009 Elsevier Inc. All rights reserved. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Basic fibroblast growth factor | en |
dc.subject | Differentiation | en |
dc.subject | Fibroblast growth factor receptor | en |
dc.subject | Glial fibrillary acidic protein | en |
dc.subject | Microglia | en |
dc.subject | Microtubule-associated protein 2 | en |
dc.title | Basic fibroblast growth factor promotes the generation of microtubule-associated protein 2-positive cells from microglia | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA00564395 | - |
dc.identifier.jtitle | Biochemical and Biophysical Research Communications | en |
dc.identifier.volume | 390 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 1018 | - |
dc.identifier.epage | 1022 | - |
dc.relation.doi | 10.1016/j.bbrc.2009.10.100 | - |
dc.textversion | author | - |
dc.identifier.pmid | 19854155 | - |
dcterms.accessRights | open access | - |
dc.identifier.pissn | 0006-291X | - |
出現コレクション: | 学術雑誌掲載論文等 |
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