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ファイル | 記述 | サイズ | フォーマット | |
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j.freeradbiomed.2009.09.0271.pdf | 534.05 kB | Adobe PDF | 見る/開く |
タイトル: | Control of hypoxia-induced tumor cell adhesion by cytophilic human catalase. |
著者: | Yata, Tomoya Nishikawa, Makiya Nishizaki, Chika Oku, Masahide https://orcid.org/0000-0001-5991-6281 (unconfirmed) Yurimoto, Hiroya https://orcid.org/0000-0001-7506-6184 (unconfirmed) Sakai, Yasuyoshi Takakura, Yoshinobu https://orcid.org/0000-0002-7359-2647 (unconfirmed) |
著者名の別形: | 西川, 元也 |
キーワード: | Abnormal cell adhesion Free radicals Hypoxia Reactive oxygen species Recombinant human catalase |
発行日: | 15-Dec-2009 |
出版者: | Elsevier BV |
誌名: | Free radical biology & medicine |
巻: | 47 |
号: | 12 |
開始ページ: | 1772 |
終了ページ: | 1778 |
抄録: | Hypoxia-induced reactive oxygen species (ROS)-mediated expression of a variety of genes in endothelial cells has been suggested to be involved in abnormal cell adhesion. To prevent this by accelerated binding of catalase to endothelial cells, human catalase (hCAT), an enzyme catalyzing the decomposition of hydrogen peroxide, was fused with three repeats of arginine-glycine-aspartic acid peptide or nona arginine peptide at the C-terminal to obtain hCAT-(RGD)3 and hCAT-R9, respectively. Human CAT and its derivatives were expressed in yeast Pichia pastoris and purified. The specific activity and secondary structure of hCAT-(RGD)3 and hCAT-R9 were close to those of hCAT, but these derivatives showed higher binding to the mouse aortic vascular endothelial cell line MAEC than hCAT, indicating that they are cytophilic derivatives. Hypoxic treatment of MAEC increased the intracellular ROS level, the binding of mouse melanoma cells, and the activity of transcription factors, hypoxia inducible factor-1 and nuclear factor-kappaB. hCAT-(RGD)3 or hCAT-R9 efficiently inhibited these changes compared with hCAT. These results indicate that cytophilic hCAT-(RGD)3 and hCAT-R9 are effective in inhibiting hypoxia-induced tumor cell adhesion to endothelial cells. |
著作権等: | c 2009 Elsevier Inc. All rights reserved. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/91262 |
DOI(出版社版): | 10.1016/j.freeradbiomed.2009.09.027 |
PubMed ID: | 19804819 |
出現コレクション: | 学術雑誌掲載論文等 |
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