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タイトル: | Sperm preservation by freeze-drying for the conservation of wild animals. |
著者: | Kaneko, Takehito Ito, Hideyuki Sakamoto, Hidefusa Onuma, Manabu Inoue-Murayama, Miho |
著者名の別形: | 金子, 武人 |
発行日: | 19-Nov-2014 |
出版者: | Public Library of Science |
誌名: | PloS one |
巻: | 9 |
号: | 11 |
論文番号: | e113381 |
抄録: | Sperm preservation is a useful technique for the maintenance of biological resources in experimental and domestic animals, and in wild animals. A new preservation method has been developed that enables sperm to be stored for a long time in a refrigerator at 4°C. Sperm are freeze-dried in a solution containing 10 mM Tris and 1 mM EDTA. Using this method, liquid nitrogen is not required for the storage and transportation of sperm. We demonstrate that chimpanzee, giraffe, jaguar, weasel and the long-haired rat sperm remain viable after freeze-drying. In all species, pronuclei were formed after the injection of freeze-dried sperm into the mouse oocytes. Although preliminary, these results may be useful for the future establishment of "freeze-drying zoo" to conserve wild animals. |
著作権等: | © 2014 Kaneko et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
URI: | http://hdl.handle.net/2433/192770 |
DOI(出版社版): | 10.1371/journal.pone.0113381 |
PubMed ID: | 25409172 |
出現コレクション: | 学術雑誌掲載論文等 |
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