泌尿器科領域の蛋白質をめぐる2,3の問題 第2編:泌尿生殖器系疾患の血漿蛋白質のポーラログラフ的研究

Abstract

Since the principle of polarography was deviced by H eyrovskY. and Shikata in 1924, extensive investigations have been undertaken in the fields of organic and inorganic chemistry as well as medicine. Brdieka in 1933 described the albumin wave by means of electrolysis of protein in Cobalt-salt solution, thence the polarographic albumin wave has been widely employed in the etiologic study of diseases or an aid of their diagnosis. In the present study polarography was employed in 149 patients with gen i t o-urinary diseases. 1. Method a) First reaction : 0.5cc of distilled water was added to 0.5cc of the oxalated plasma and it was treated with 0.25cc of 1N KOH. Then the wave was obtained with 0.5cc of this solution by the polarographic apparatus using Co＋＋ salt buffer solution. b) Second reaction One cc of distilled water was added to 0.5cc of the oxalated plasma and it was treated with 0.1cc of 1N KOH. After leave this solution alone for a constant time, lcc of 2% sulfosalicilic acid was added, and its protein was precipitated by Watman No. 5 filter paper. The wave was then obtained with 0.05cc of this solution by the polarographic apparatus using Co＋＋ salt buffer solution . 2. Chemical treatment of the plasma was performed fo r 30 minutes at a temperature between 10° and 25°C. 3. Normal pla s ma albumin wave was shown in Figure 2. 4. The plasma albumine waves of the patients were show n in Figures 3-12. 5. Conclusion : Although a change in the polarographic albumin wav e was not specific in a particular disease, the more significant changes that the wave was depressed in the first reaction, while it was elevated in the second reaction were obtained in a group of patients with tumor than in a group of patients without tumor. The changes of the wave were more significant in a group of patients with generalized systemic disease than in a group of patients with localized disesse. The polarography, therefore, is applicable to a supplementary diagnostic procedure from the changes of the waves in both first and second reactions. Read at 7th meeting of Chubu Section of Japan Urological Association in Kyoto University, Novenber, 1956 and at 45th general meeting of Japan Urological Association in Nagoya University, April, 1957.