非淋菌性尿道炎に於けるPleuropneumonia-LikeOrganisms(PPLO)に関する知見補遺 第1編:PPLO分離のためのThalliumAcetate含有選択培地に就て

Abstract

1) Three strainsof PPLO isolated from male non-gonococcal urethritis (NGU) and 7 species of bacteriawere examined to determinate the effects of thallium acetate in "PPLO agar (Eiken)" in suppressing their growth . The results showed that ; The three strains of PPL O were not inhibited by thallium acetate in a 1 500 concentration, but the strains of Corynebacterium and Gaffkya tetragena were inhibited by a 1 2, 500 concentration. Conce n trations of 1 : 1, 000 of thallium acetate inhibited the growth of Neisseria gonorrhoeae and Serratia marcescens, while the strains of Escherichia coli were inhibited by a 1 500 concentration. Gra m positive diplococcus (isolated from NGU) was the only bacteria which appeared to be uneffected by the thallium acetate. No "L" type colonies were o bserved on any of the thallium acetate plates inoculated with bacteria. 2) At the Urological Department of the Communications Hospital, Osaka, from 1955 to 1958, a total of 450 patients were studied to evaluate a selective medium containing thallium acetate for the isolation of PPLO from clinical material. PPLO were isolated from 44 out of 450 cases ; 28 ( 12.2 per cent) of 230 male cases with NGU, 4 (3.1 per cent) of 131 gonococcal urethritis, 2 (3.5 per cent) of 56 healthy men and 10 (30.3 per cent) of 33 gynecologic patients. Of a total of 44 strains of PPLO, 36 (82 per cent) grew on a selective medium containing of 0.04 per cent of thallium acetate, including 29 strains (80 per cent) that were isolated in pure cultures. Of 44 strains, 24 were recovered on "PPLO agar (Eiken)" of which 6 (25 pet cent) were isolated in pure culture. On the "Ishihara m edium" for gonococci, PPLO were isolated in pure culture in 7 (29 per cent) out of 24 cases with positive cultures. The selective medium containing t h allium acetate was most effective in inhibiting contaminants as compared with "PPLO agar (Eiken)" and "Ishihara medium" for gonococci, and thus has proven very useful for the isolation of PPLO from clinical material.