沢瀉および夏枯草のラットにおける蓚酸カルシウム結石形成に対する作用

Abstract

1)ラットを用いた蓚酸カルシウム結石実験モデルを用いて猪苓湯の成分である沢瀉を投与し, 結石形成抑制作用があることが認められた. 2)in vitroの実験系で強い抑制作用を認めた夏枯草は, このラット結石実験モデルにおいては結石形成の抑制作用はなかった. 3)沢瀉は蓚酸, およびカルシウム代謝に影響を与えることなく, ラット結石実験モデルにおいて結石形成抑制作用を示した

We examined the inhibitory effect of the two Kampo medicines, takusha and kagosou on the formation of calcium oxalate renal stones induced by ethylene glycol (EG) and 1 alpha(OH)D3 (1 alpha-D3) in rats. Wistar strain rats were divided into 4 groups (A: normal control, B: stone, C: kagosou, D: takusya). There was no significant difference in urinary calcium excretion or oxalate excretion between the stone group and kampo medicine groups. The calcium content of the kidneys was significantly lower in the takusha group than in the other two groups (Stone group and kagosou group). Takusha was effective in preventing oxalate stone formation in rats. Kagosou, which had strong inhibitory effect on calcium oxalate crystal growth and aggregation in vitro as well as takusha, was not effective against in vivo calcium oxalate stone formation in rats. These findings suggest that takusha prevents the formation of calcium oxalate stone by inhibiting calcium oxalate crystal growth and aggregation.