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Title: 尿中分離細菌のウレアーゼ活性
Other Titles: Urease activity of bacteria in urine
Authors: 新井, 豊  KAKEN_name
竹内, 秀雄  KAKEN_name
友吉, 唯夫  KAKEN_name
立脇, 憲一  KAKEN_name
Author's alias: ARAI, Yutaka
TAKEUCHI, Hideo
TOMOYOSHI, Tadao
TATEWAKI, Kenichi
Keywords: Urease activity
Bacteria
Urinary stone
Issue Date: Feb-1989
Publisher: 泌尿器科紀要刊行会
Journal title: 泌尿器科紀要
Volume: 35
Issue: 2
Start page: 277
End page: 281
Abstract: 1)尿中細菌のウレアーゼ活性の陽性率を調べると,Proteus, Morganella, Klebsiellaで90%以上の株で陽性であり,E. coliは全例陰性であった.球菌についても陽性をしめすものがあった.2)各細菌のウレアーゼ活性を定量的に調べた.M. morganii, S. epidermidisに強い活性があった.P. mirabilis, K. pneumoniae, P. aeruginosaは中等度の活性を示した.E. coliは陰性であった.またS. epidermidisは株により,強い活性から陰性のものまであった.3)ヒト尿にウレアーゼ産生菌を混入し,尿pHの変化を調べた.P. mirabilis接種症で著しい尿pHの上昇があり,ウレアーゼ活性が強いことが示唆された.またM. morganii, S. epidermidisにも尿pH上昇を認めたが,P. aeruginosaでは尿pHは上昇しなかった.4)細菌のウレアーゼ活性定量と尿アルカリ化作用は必ずしも一致しなかった.これは各細菌の至適pHの相違および反応時間によるものと推測された
Urea splitting bacteria are related to the formation of struvite or apatite. We investigated the urease activity of bacteria by two methods; the direct measurement of urease activity of viable bacteria and sonicated bacteria from amounts of ammonia by the indophenol method, and the measurement of urease activity by alkalization of infected urine. Proteus mirabilis and Pseudomonas aeruginosa had moderate activity of urease, and Morganella morganii and Staphylococcus epidermidis had the most powerful activity. P. mirabilis caused the strongest alkalization in infected urine.
URI: http://hdl.handle.net/2433/116435
PubMed ID: 2500012
Appears in Collections:Vol.35 No.2

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