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Title: フローサイトメトリーによる前立腺上皮細胞のPA(前立腺特異抗原)/DNA量同時解析
Other Titles: Prostate-specific antigen (PA) and deoxyribonucleic acid (DNA) analysis in prostatic epithelial cells using flow cytometry
Authors: 島袋, 智之  KAKEN_name
吉弘, 悟  KAKEN_name
松山, 豪泰  KAKEN_name
山本, 憲男  KAKEN_name
酒徳, 治三郎  KAKEN_name
Author's alias: Shimabukuro, Tomoyuki
Yoshihiro, Satoru
Matsuyama, Hideyasu
Yamamoto, Norio
Sakatoku, Jisaburo
Keywords: Aged
Antigens, Neoplasm
Epithelial Cells
Flow Cytometry/methods
Middle Aged
Prostate-Specific Antigen
Prostatic Hyperplasia/pathology
Issue Date: Aug-1989
Publisher: 泌尿器科紀要刊行会
Journal title: 泌尿器科紀要
Volume: 35
Issue: 8
Start page: 1335
End page: 1337
Abstract: 前立腺上皮細胞内PA量とDNA量を同時に測定可能なFCM法を開発した.その可能性を探るため,前立腺肥大症の組織検体を用いて基礎的検討を行った.1) FCM法により,前立腺上皮細胞内PA量とDNA量を同時に測定できた.2)前立腺肥大症の全例ともDNA分布はdiploid patternを呈していた.3) PAは2CのDNA量を有する細胞(G0,1期細胞)でのみ産生され,S期やG2M期にある細胞では産生されていなかった
We developed a new method to analyze simultaneously cellular prostate-specific antigen (PA) content and deoxyribonucleic acid (DNA) content of each prostatic epithelial cell by using flow cytometry (FCM). We report the possibilities of this method and we analyzed firstly 8 samples from 6 patients with benign prostatic hypertrophy (BPH). The content of cellular PA and DNA of prostatic epithelial cells can be analyzed simultaneously. The PA is synthesized only in the cells with a 2C DNA content (G0/1 cells) and not in the S- or G2M-phase cells. In the near future, by applying this analytical method to prostatic cancer samples we could obtain more interesting knowledge of the behavior of prostatic cancer.
PubMed ID: 2479235
Appears in Collections:Vol.35 No.8

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