各種漢方成分による蓚酸カルシウム結晶成長凝集阻止能の検討

Abstract

1)各種漢方成分のなかには, in vitroにて強い結晶成長凝集阻止能を持つものが存在した.2)今回実験に用いた7成分中, 夏枯草および沢瀉に低濃度においても, 強い阻止能を認め, 結石予防薬としての可能性が示唆された.3)各種エキスの阻止能とABPPとの間に相関は認められず, とくに沢瀉の成分中にはGAG以外の阻止物質の存在が示唆された

The effects of the compositional drugs of kanpou medicine on the growth and aggregation of calcium oxalate crystals were investigated in vitro. In the seeded crystal system, the inhibitory activities were calculated from the change of the number (Ia), total volume (Ig) and fractional volume (I) of crystals determined by a Coulter Multisizer. Among the tested drugs (Takusha, Akyou, Chorei, Bukuryou, Kasseki, Kinsensou, Kagosou), only Takusha and Kagosou had strong inhibitory activities on the growth and aggregation at the concentration of 5 micrograms/ml and 10 micrograms/ml (Ia greater than 80%, Ig, I greater than 90%). No significant correlation was observed between inhibitory activities and alcian blue precipitable polyanions (ABPP) of each drug. Thus some naturally existing substances composing the kanpou medicine may help prevent the stone formation in clinical use.