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Title: ヒト前立腺組織特異抗原の研究 : 未知の抗原に対するモノクローナル抗体作製法のモデル
Other Titles: Human prostatic tissue specific antigens: a model for the research of monoclonal antibodies against unknown antigens
Authors: 吉貴, 達寛  KAKEN_name
岡田, 裕作  KAKEN_name
友吉, 唯夫  KAKEN_name
上田, 正道  KAKEN_name
小川, 修  kyouindb  KAKEN_id
吉田, 修  KAKEN_name
Author's alias: Yoshiki, Tatsuhiro
Okada, Yusaku
Tomoyoshi, Tadao
Ueda, Masamichi
Ogawa, Osamu
Yoshida, Osamu
Keywords: Prostate-associated antigen
Monoclonal antibody
Issue Date: Mar-1993
Publisher: 泌尿器科紀要刊行会
Journal title: 泌尿器科紀要
Volume: 39
Issue: 3
Start page: 213
End page: 219
Abstract: 前立腺分泌細胞,基底細胞,間質細胞などの混合物を免疫原にし,BPH切片に対する蛍光抗体法でスクリーニングしてマウスモノクローナル抗体を作製した。前立腺分泌細胞に良く反応し,ホルマリン固定パラフィン切片にも適用可能である,PAP,PSAにそれぞれ特異的な抗体を産生する細胞を1クローンずつ樹立できた
Mouse monoclonal antibodies were prepared using a cell mixture containing prostatic secretory, basal cells and stromal cells as an immunogen, and by the immunofluorescence method with cryosections of prostate tissue. An immunohistochemical screening process was used in the expectation that it would help identify not only the antibodies reacting with the secretory cells predominant in the prostate but also those reacting with relatively rare cells in the prostate tissue. Two cell clones have been established which produce antibodies (IgG1) that react well with prostatic secretory cells: a clone producing antibodies specific to prostatic acid phosphatase (PAP) and one producing antibodies specific to prostate-specific antigen (PSA). These antibodies could recognize the native antigens in the seminal plasma, respectively. These findings indicate that antibodies recognizing only one substance can be prepared, even when a mixture of prostatic tissue including many proteases is used as an immunogen. Production of antibodies against prostate-specific antigens other than PAP and PSA by this method should provide a useful means for analyzing unknown antigens in the prostate.
URI: http://hdl.handle.net/2433/117806
PubMed ID: 7685139
Appears in Collections:Vol.39 No.3

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