Human Papillomavirus DNAの検出を行った尿道尖圭コンジローマの1例

Abstract

35歳男, 尿道尖圭コンジローマの1例を経験し, 腫瘍切除術および電気凝固術を施行した。また腫瘍部よりDNAを抽出しドットブロットハイブリダイゼーション法とサザンブロットハイブリダイゼーション法にてHPV-DNAの型を決定することができた

A case in a 35-year-old male with recurrence of the condyloma acuminatum of the urethra is presented and the involvement of human papilloma virus (HPV) with this disease is mentioned. The HPV-DNA in the excised specimen was detected by the dot blot hybridization method with radiolabelled RNA probes, and the type of the DNA was investigated by the Southern blot hybridization method with biotinylated DNA probes, which could detect the HPV-DNA of types 6, 11, 16, 18, 31, 33 and 35. These analyses demonstrated the HPV-DNA of type 11 and another unidentified type in our case. From the literature with regard to the etiology of this disease considering the risk of malignancy, we also emphasize the necessity for close observation of the patients with condyloma acuminatum, clinically benign, which contains the so-called malignant type HPV-DNA.