前立腺癌の軟寒天培養 --とくにtestosterone添加の影響について--

Abstract

Stem cell assay法を用いてヒト前立腺癌細胞の二層軟寒天培養を検討し, 14例中9例にコロニー形成を認めた.とくにリンパ節より得られた組織で高いコロニー形成率が認められ, 各種ホルモン添加の影響を検討できた.testosterone添加の検体の中にはコロニー形成の増大を示すものがあり, 臨床的に抗アンドロジェン療法に対する反応と相関を示すと考えられるものがあった

Soft agar cultures of human prostatic carcinoma cells obtained from prostates with needle biopsy and metastatic lymph nodes were examined. Colony formation were observed in 2 of the 4 needle biopsy specimens and in 7 of the 10 lymphatic nodes specimens. The plating efficiency was 0.04 to 0.43%. The effect of testosterone on the clonal growth of these cells in soft agar culture was studied. In half of the cases, colony formation was increased by the addition of testosterone. Whereas in the other cases, colony formation was not influenced by the testosterone. Clinically, most of the former cases responded well to the anti-androgen therapy. Neither EGF nor hydrocortisone showed any effect on the colony formation of the prostatic cancer cells in soft agar. These results suggest that primary culture of human prostatic cancer is possible in soft agar medium, and that androgen dependency of these cells can be examined in vitro.