Thomsen-Friedenreich antigen in bladder cancer tissues detected by monoclonal antibody

Abstract

73例の膀胱癌組織標本においてT抗原の有無をモノクローナル抗体による免疫蛍光組織法で検索した.悪性度が低い腫瘍では17例(53%), 中等度の腫瘍では18例(44%), 悪性度の高い腫瘍では5例(50%)でT-陽性であり, 悪性度と陽性率に差を認めなかった.T-陰性の腫瘍のうち, ノイラミニダーゼ処理後には悪性度が低い腫瘍で14例(44%)中等度の腫瘍で13例(32%)が陽性であったのに対し, 悪性度が高度の腫瘍では僅かに1例(10)がcryptic T-陽性であった.逆に, ノイラミニダーゼ処理後にもT-陰性であったのは, 悪性度の低い例で1例(3%)であるのに対し, 中等度悪性例では10例(24%), 高度悪性例では4例(40%)と, 組織学的悪性度とT抗原の表現形式には有意の相関がみられた.また, T-陽性群では28例(70%), cryptic T-陰性群では9例(60%)に再発をみたのに対し, cryptic T-陽性例では3例(11%)でしか再発しなかった

Monoclonal antibodies against Thomsen-Friedenreich antigen (T-Ag) were obtained by the hybridoma technique. The expression of T-Ag in 73 specimens of bladder cancer was examined by the immunofluorescence method using these monoclonal antibodies. Seventeen (53%) of grade I, 18 (44%) of grade II and 5 (50%) of grade III were diffusely stained with anti-T. Of the T-negative tumors, 14 (44%) of grade I and 13 (32%) of grade II showed positive staining after neuraminidase treatment (cryptic T-positive) while only 1 (10%) of grade III was cryptic T-positive. On the contrary, only 1 case (3%) of grade I was cryptic T-negative while 10 (24%) of grade II and 4 (40%) of grade III (were. The T-antigen expression and the histological grades correlated significantly (p less than 0.05). Twenty-eight (70%) of the T-positive cases and 9 (60%) of the cryptic T-negative cases recurred while only 3 (11%) of the cryptic T-positive cases did (p less than 0.01). The monoclonal antibodies were more useful than peanut lectin (PNA) or conventional polyclonal antibodies on the detection of T-antigen.