ヒト精巣FSH レセプター測定法について

Abstract

1) Human FSHのradioiodinationをlactoperoxidase法にて行い, ヒト精巣FSHレセプター測定に用いた.2)ヒト精巣FSHレセプターは10 mg相当の精巣ホモゲネートを25°Cで20時間, 種々の濃度の〔125I〕human FSHとincubateすることにより測定できた.3) Scatchard analysisにてFSHレセプターのKaとBmaxを求めた.10例の未治療前立腺癌患者の精巣はいずれもaffinityの異なる2つのbinding siteをもっていた

We established the assay conditions for human testicular FSH receptors using testicular tissues obtained from ten patients with prostate cancer. Based on the results of the time course study on specific FSH binding with increasing concentrations of the testicular homogenate at various temperatures, we found that the incubation was suitable at 25 degrees C for 20 hours using 10 mg wet tissue per tube. Scatchard analysis of specific FSH binding sites showed that FSH receptors in the human testicular tissue consisted of two classes of binding sites, one with a high affinity (Ka, 10(9) M-1) and the other with a low affinity (Ka, 10(8) M-1).