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タイトル: | Human tumor colony forming assayによる抗癌剤感受性試験について |
その他のタイトル: | In vitro chemosensitivity test by human tumor colony forming assay |
著者: | 金丸, 洋史 ![]() 橋村, 孝幸 ![]() 筧, 善行 ![]() 李, 南杢 ![]() 吉田, 修 ![]() |
著者名の別形: | KANAMARU, Hiroshi HASHIMURA, Takayuki KAKEHI, Yoshiyuki LEE, Nam Kyu YOSHIDA, Osamu |
キーワード: | Urological cancer Chemosensitivity test Human tumor colony forming assay 'H-thymidine incorporation assay |
発行日: | Nov-1988 |
出版者: | 泌尿器科紀要刊行会 |
誌名: | 泌尿器科紀要 |
巻: | 34 |
号: | 11 |
開始ページ: | 1917 |
終了ページ: | 1921 |
抄録: | In vitro chemosensitivity of urological cancers was assessed by a human tumor colony forming assay (HTCA) and a 3H-thymidine incorporation assay. Primary tumor cells from 160 of 253 (63%) urological cancers showed adequate colony growth (greater than 30 colonies per well), and there was a 57% true positive and 100% true negative rate for predicting clinical response of anticancer agents. On the other hand, cells from 37 of 45 (82%) urologic cancers incorporated a sufficient amount of 3H-thymidine (greater than 300 cpm). However, when the positive control drug (chromomycin A3 100 micrograms/ml) was used for the assay quality control, the successful assay rate of the HTCA (38%) was lower than that of the 3H-thymidine incorporation assay (75%), while there was a significant correlation in drug sensitivities between the two assays. Thus, the 3H-thymidine incorporation assay seemed to be more useful than the HTCA for evaluating the chemosensitivity of urologic cancers. |
URI: | http://hdl.handle.net/2433/119779 |
PubMed ID: | 3242365 |
出現コレクション: | Vol.34 No.11 |
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