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Title: Detection of cesA mRNA from Bacillus cereus by RNA-specific amplification
Authors: Yasukawa, Kiyoshi  kyouindb  KAKEN_id
Agata, Norio
Inouye, Kuniyo
Author's alias: 保川, 清
Keywords: Bacillus cereus
Cereulide
PCR
RNA-specific amplification
RT-PCR
Issue Date: 5-Apr-2010
Publisher: Elsevier
Journal title: Enzyme and Microbial Technology
Volume: 46
Issue: 5
Start page: 391
End page: 396
Abstract: Cereulide is the cyclic dodecadepsipeptide responsible for the emetic-type food-borne disease caused by Bacillus cereus. It is synthesized enzymatically by non-ribosomal peptide synthetases. In this study, we established an RNA-specific amplification assay for the detection of mRNA transcribed from the cesA gene, one of the 7 genes constituting the cereulide synthetase (ces) gene cluster of emetic-type B. cereus. The assay detected as few as 1000 copies of this RNA. Examination of the total RNAs extracted from 7 strains of cereulide-producing B. cereus, 6 strains of cereulide non-producing B. cereus, 6 strains of other Bacillus species, and 10 non-Bacillus strains showed that the assay specifically detected emetic-type B. cereus strains. RNA-specific amplification using RNA extracts provided comparable results to RT-PCR assays using RNA extracts and PCR assays using DNA extracts. The RNA-specific amplification assay is suitable for the detection of mRNA transcribed from the cesA gene.
Rights: © 2010 Elsevier B.V.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/120342
DOI(Published Version): 10.1016/j.enzmictec.2009.12.009
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