尿中トリプシンインヒビター(UTI)に関する研究 -UTIの新定量法について-

Abstract

A new determination of urinary trypsin inhibitor (UTI) was developed from casein-plate method. We studied the relationship between the activity level of UTI by the present method and the activity level of urokinase (UK) by fibrin agar-plate method in various renal diseases. A sample of trypsin solution (Sigma, type II, 25 mM Ca2+) was adsorbed to a filter paper (5.8×5.8 mm), and lyophilized to be fixed. Then 5 pi of urine was adsorbed to the above. It was applied on 0.05% casein-plate (10 mM Ca2+), and incubated at 37"C. UTI activity level was determined in lysis area of trypsin. The results were well correlated with those by the test-tube method already reported. The procedure of the present method made it possible to test more simply compared with conventional methods. The amount to inhibit 1 pg of trypsin was assigned to be one unit, as for UTI activity. As for UK level in urine, UK was determined by fibrin agar-plate method with commercial UK (Green Cross Co. Ltd.) as the standard. The results are as follows: 1. Normal human urine contains about 4.1 units of UTI and 11.1 IU of UK per ml. UTI/UK is approximately 0.4.