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Title: Promotion of direct reprogramming by transformation-deficient Myc
Authors: Nakagawa, Masato  kyouindb  KAKEN_id  orcid (unconfirmed)
Takizawa, Nanako
Narita, Megumi
Ichisaka, Tomoko
Yamanaka, Shinya  kyouindb  KAKEN_id
Author's alias: 中川, 誠人
山中, 伸弥
Keywords: induced pluripotent stem cell
embryonic stem cell
regenerative medicine
Issue Date: 26-Jul-2010
Publisher: The National Academy of Sciences
Journal title: Proceedings of the National Academy of Sciences
Volume: 107
Issue: 32
Start page: 14152
End page: 14157
Abstract: Induced pluripotent stem cells (iPSCs) are generated from mouse and human fibroblasts by the introduction of three transcription factors: Oct3/4, Sox2, and Klf4. The proto-oncogene product c-Myc markedly promotes iPSC generation, but also increases tumor formation in iPSC-derived chimeric mice. We report that the promotion of iPSC generation by Myc is independent of its transformation property. We found that another Myc family member, L-Myc, as well as c-Myc mutants (W136E and dN2), all of which have little transformation activity, promoted human iPSC generation more efficiently and specifically compared with WT c-Myc. In mice, L-Myc promoted germline transmission, but not tumor formation, in the iPSC-derived chimeric mice. These data demonstrate that different functional moieties of the Myc proto-oncogene products are involved in the transformation and promotion of directed reprogramming.
Description: 形質転換活性を欠損したMycによるリプログラミング促進効果:L-Mycを用いた効率的なiPS細胞の樹立. 京都大学プレスリリース. 2010-07-27.
Rights: © 2010 the National Academy of Sciences
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1073/pnas.1009374107
PubMed ID: 20660764
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