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dc.contributor.authorAn, Mengnanen
dc.contributor.authorIwakawa, Hiro-okien
dc.contributor.authorMine, Akiraen
dc.contributor.authorKaido, Masanorien
dc.contributor.authorMise, Kazuyukien
dc.contributor.authorOkuno, Tetsuroen
dc.contributor.alternative奥野, 哲郎ja
dc.date.accessioned2010-10-18T05:51:29Z-
dc.date.available2010-10-18T05:51:29Z-
dc.date.issued2010-09-15-
dc.identifier.issn1096-0341-
dc.identifier.urihttp://hdl.handle.net/2433/128764-
dc.description.abstractRed clover necrotic mosaic virus (RCNMV) is a positive-strand RNA virus with a bipartite genome. RNA1 encodes N-terminally overlapping replication proteins, p27 and p88. RNA2 is replicated efficiently by the replication proteins supplied in trans, whereas RNA1 needs p88 preferentially in cis for its replication. cis-Acting elements required for RNA2 replication have been mapped to the 3' terminal stem-loop structure conserved between RNA1 and RNA2, and to the protein-coding region including the trans-activator. Here, we have identified a Y-shaped RNA structure with three-way RNA junctions predicted in the 3' untranslated region of RNA2 as a novel element required for negative-strand synthesis using an in vitro translation/replication system. We also show that, in addition to the 3' terminal core promoter, several RNA elements including the trans-activator are also required for negative-strand synthesis. Functional roles and structural requirements of these cis-acting elements in RCNMV RNA replication are discussed.en
dc.format.mimetypeapplication/pdf-
dc.language.isoeng-
dc.publisherElsevier Inc.en
dc.rights© 2010 Elsevier Inc.en
dc.rightsこの論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.rightsThis is not the published version. Please cite only the published version.en
dc.subjectRNA replicationen
dc.subjectRNA virusen
dc.subjectNegative-strand RNA synthesisen
dc.subjectY-shaped RNA structureen
dc.subjectTrans-activatoren
dc.subjectMultifunctional RNAen
dc.subjectPlant virusen
dc.subjectTombusviridaeen
dc.subjectDianthovirusen
dc.subject.mesh3' Untranslated Regionsen
dc.subject.meshGene Expression Regulation, Viral/physiologyen
dc.subject.meshMutationen
dc.subject.meshNucleic Acid Conformationen
dc.subject.meshPromoter Regions, Genetic/geneticsen
dc.subject.meshPromoter Regions, Genetic/physiologyen
dc.subject.meshRNA, Viral/biosynthesisen
dc.subject.meshRNA, Viral/geneticsen
dc.subject.meshRNA, Viral/metabolismen
dc.subject.meshTombusviridae/geneticsen
dc.subject.meshTombusviridae/metabolismen
dc.subject.meshTrans-Activators/geneticsen
dc.subject.meshTrans-Activators/metabolismen
dc.subject.meshViral Proteins/geneticsen
dc.subject.meshViral Proteins/metabolismen
dc.titleA Y-shaped RNA structure in the 3' untranslated region together with the trans-activator and core promoter of Red clover necrotic mosaic virus RNA2 is required for its negative-strand RNA synthesis.en
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.ncidAA00884454-
dc.identifier.jtitleVirologyen
dc.identifier.volume405-
dc.identifier.issue1-
dc.identifier.spage100-
dc.identifier.epage109-
dc.relation.doi10.1016/j.virol.2010.05.022-
dc.textversionauthor-
dc.identifier.pmid20561661-
dcterms.accessRightsopen access-
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