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Title: A Y-shaped RNA structure in the 3' untranslated region together with the trans-activator and core promoter of Red clover necrotic mosaic virus RNA2 is required for its negative-strand RNA synthesis.
Authors: An, Mengnan
Iwakawa, Hiro-oki
Mine, Akira  kyouindb  KAKEN_id  orcid (unconfirmed)
Kaido, Masanori  KAKEN_id
Mise, Kazuyuki  kyouindb  KAKEN_id  orcid (unconfirmed)
Okuno, Tetsuro  KAKEN_id
Author's alias: 奥野, 哲郎
Keywords: RNA replication
RNA virus
Negative-strand RNA synthesis
Y-shaped RNA structure
Multifunctional RNA
Plant virus
Issue Date: 15-Sep-2010
Publisher: Elsevier Inc.
Journal title: Virology
Volume: 405
Issue: 1
Start page: 100
End page: 109
Abstract: Red clover necrotic mosaic virus (RCNMV) is a positive-strand RNA virus with a bipartite genome. RNA1 encodes N-terminally overlapping replication proteins, p27 and p88. RNA2 is replicated efficiently by the replication proteins supplied in trans, whereas RNA1 needs p88 preferentially in cis for its replication. cis-Acting elements required for RNA2 replication have been mapped to the 3' terminal stem-loop structure conserved between RNA1 and RNA2, and to the protein-coding region including the trans-activator. Here, we have identified a Y-shaped RNA structure with three-way RNA junctions predicted in the 3' untranslated region of RNA2 as a novel element required for negative-strand synthesis using an in vitro translation/replication system. We also show that, in addition to the 3' terminal core promoter, several RNA elements including the trans-activator are also required for negative-strand synthesis. Functional roles and structural requirements of these cis-acting elements in RCNMV RNA replication are discussed.
Rights: © 2010 Elsevier Inc.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.virol.2010.05.022
PubMed ID: 20561661
Appears in Collections:Journal Articles

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