|Title:||Positive and negative cooperativity of modularly assembled zinc fingers.|
|Authors:||Imanishi, Miki https://orcid.org/0000-0002-4172-0966 (unconfirmed)|
Futaki, Shiroh https://orcid.org/0000-0002-0124-4002 (unconfirmed)
|Author's alias:||今西, 未来|
|Keywords:||Engineered zinc finger protein|
|Journal title:||Biochemical and biophysical research communications|
|Abstract:||One simple and widespread method to create engineered zinc fingers targeting the desired DNA sequences is to modularly assemble multiple finger modules pre-selected to recognize each DNA triplet. However, it has become known that a sufficient DNA binding affinity is not always obtained. In order to create successful zinc finger proteins, it is important to understand the context-dependent contribution of each finger module to the DNA binding ability of the assembled zinc finger proteins. Here, we have created finger-deletion mutants of zinc finger proteins and examined the DNA bindings of these zinc fingers to clarify the contributions of each finger module. Our results indicate that not only a positive cooperativity but also a context-dependent reduction in the DNA binding activity can be induced by assembling zinc finger modules.|
|Rights:||© 2009 Elsevier Inc.|
This is not the published version. Please cite only the published version.
|Appears in Collections:||Journal Articles|
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