Downloads: 329

Files in This Item:
File Description SizeFormat 
j.bbrc.2009.07.059.pdf294 kBAdobe PDFView/Open
Title: Positive and negative cooperativity of modularly assembled zinc fingers.
Authors: Imanishi, Miki  kyouindb  KAKEN_id  orcid (unconfirmed)
Nakamura, Atsushi
Morisaki, Tatsuya
Futaki, Shiroh  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 今西, 未来
Keywords: Engineered zinc finger protein
DNA binding
Modular assembly
Issue Date: 25-Sep-2009
Publisher: Elsevier
Journal title: Biochemical and biophysical research communications
Volume: 387
Issue: 3
Start page: 440
End page: 443
Abstract: One simple and widespread method to create engineered zinc fingers targeting the desired DNA sequences is to modularly assemble multiple finger modules pre-selected to recognize each DNA triplet. However, it has become known that a sufficient DNA binding affinity is not always obtained. In order to create successful zinc finger proteins, it is important to understand the context-dependent contribution of each finger module to the DNA binding ability of the assembled zinc finger proteins. Here, we have created finger-deletion mutants of zinc finger proteins and examined the DNA bindings of these zinc fingers to clarify the contributions of each finger module. Our results indicate that not only a positive cooperativity but also a context-dependent reduction in the DNA binding activity can be induced by assembling zinc finger modules.
Rights: © 2009 Elsevier Inc.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.bbrc.2009.07.059
PubMed ID: 19615972
Appears in Collections:Journal Articles

Show full item record

Export to RefWorks

Export Format: 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.