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j.antiviral.2008.05.006.pdf299.39 kBAdobe PDF見る/開く
タイトル: Novel screening systems for HIV-1 fusion mediated by two extra-virion heptad repeats of gp41.
著者: Nishikawa, Hiroki
Kodama, Eiichi
Sakakibara, Ayako
Fukudome, Ayako
Izumi, Kazuki
Oishi, Shinya  kyouindb  KAKEN_id
Fujii, Nobutaka  KAKEN_id
Matsuoka, Masao  KAKEN_id
著者名の別形: 大石, 真也
児玉, 栄一
キーワード: HIV-1
Gp41
Fusion
ELISA
Screening
Alkaline phosphatase
発行日: Oct-2008
出版者: Elsevier
誌名: Antiviral research
巻: 80
号: 1
開始ページ: 71
終了ページ: 76
抄録: Entry of human immunodeficiency virus type 1 (HIV-1) into target cells is mediated by its envelope protein gp41 through membrane fusion. Interaction of two extra-virion heptad repeats (HRs) in the gp41 plays a pivotal role in the fusion, and its inhibitor, enfuvirtide (T-20), blocks HIV-1 entry. To identify agents that block HIV-1 fusion, two screening methods based on detection and quantification by the enzyme-linked immunosorbent assay (ELISA) principle have been established. One method uses an alkaline phosphatase (ALP)-conjugated antibody (Ab-ELISA) and the other uses an ALP-fused HR (F-ELISA) to detect and quantify the interaction of the two HRs. The F-ELISA was more simple and rapid, since no ALP-conjugated antibody reaction was required. Both ELISAs detected all the fusion inhibitors tested except for T-20. Interaction of the two HRs was observed in both ELISAs, even in the presence of 10% dimethyl sulfoxide. Ab-ELISA performed best in a pH ranging from 6 to 8, while F-ELISA performed best at a pH ranging from 7 to 8. These results indicate that both established ELISAs are suitable for the identification of HIV-1 fusion inhibitors.
著作権等: © 2008 Elsevier B.V.
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/137211
DOI(出版社版): 10.1016/j.antiviral.2008.05.006
PubMed ID: 18584890
出現コレクション:学術雑誌掲載論文等

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