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ファイル | 記述 | サイズ | フォーマット | |
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PNAS.1104030108.pdf | 2.19 MB | Adobe PDF | 見る/開く |
タイトル: | Processive phosphorylation of ERK MAP kinase in mammalian cells. |
著者: | Aoki, Kazuhiro https://orcid.org/0000-0001-7263-1555 (unconfirmed) Yamada, Masashi Kunida, Katsuyuki Yasuda, Shuhei Matsuda, Michiyuki https://orcid.org/0000-0002-5876-9969 (unconfirmed) |
著者名の別形: | 青木, 一洋 |
キーワード: | simulation phos-tag crowder quantitative parameter EGF |
発行日: | 18-Jul-2011 |
出版者: | National Academy of Sciences |
引用: | Aoki K, Yamada M, Kunida K, Yasuda S, Matsuda M. Processive phosphorylation of ERK MAP kinase in mammalian cells. Proc Natl Acad Sci U S A. 2011 Jul 18. [Epub ahead of print] |
誌名: | Proceedings of the National Academy of Sciences of the United States of America |
巻: | 108 |
号: | 31 |
開始ページ: | 12675 |
終了ページ: | 12680 |
抄録: | The mitogen-activated protein (MAP) kinase pathway is comprised of a three-tiered kinase cascade. The distributive kinetic mechanism of two-site MAP kinase phosphorylation inherently generates a nonlinear switch-like response. However, a linear graded response of MAP kinase has also been observed in mammalian cells, and its molecular mechanism remains unclear. To dissect these input-output behaviors, we quantitatively measured the kinetic parameters involved in the MEK (MAPK/ERK kinase)-ERK MAP kinase signaling module in HeLa cells. Using a numerical analysis based on experimentally determined parameters, we predicted in silico and validated in vivo that ERK is processively phosphorylated in HeLa cells. Finally, we identified molecular crowding as a critical factor that converts distributive phosphorylation into processive phosphorylation. We proposed the term quasi-processive phosphorylation to describe this mode of ERK phosphorylation that is operated under the physiological condition of molecular crowding. The generality of this phenomenon may provide a new paradigm for a diverse set of biochemical reactions including multiple posttranslational modifications. |
記述: | 癌遺伝子情報伝達経路の実測データに基づくシミュレーションモデルの構築~コンピューターによる抗癌剤デザインに向けて~. 京都大学プレスリリース. 2011-07-19. |
著作権等: | This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/143060 |
DOI(出版社版): | 10.1073/pnas.1104030108 |
PubMed ID: | 21768338 |
関連リンク: | https://www.kyoto-u.ac.jp/static/ja/news_data/h/h1/news6/2011/110719_1.htm http://www.pnas.org/content/early/2011/07/12/1104030108.full.pdf+html |
出現コレクション: | 学術雑誌掲載論文等 |
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