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dc.contributor.author | Nakano, Shun | en |
dc.contributor.author | Nakata, Eiji | en |
dc.contributor.author | Morii, Takashi | en |
dc.contributor.alternative | 森井, 孝 | ja |
dc.date.accessioned | 2011-09-26T02:06:18Z | - |
dc.date.available | 2011-09-26T02:06:18Z | - |
dc.date.issued | 2011-08-01 | - |
dc.identifier.issn | 0960-894X | - |
dc.identifier.uri | http://hdl.handle.net/2433/147154 | - |
dc.description.abstract | A GTP aptamer was converted to a modular fluorescent GTP sensor by conjugation of RRE (Rev responsive element) RNA and successive complex formation with a fluorophore-modified Rev peptide. Structural changes associated with substrate binding in the RNA aptamer were successfully transduced into changes in fluorescence intensity because of the modular structure of ribonucleopeptides. A simple modular strategy involving conjugation of a fluorophore-modified ribonucleopeptide to the stem region of an RNA aptamer deduced from secondary structural information helps produce fluorescent sensors, which allow tuning of excitation and detection wavelengths through the replacement of the fluorophore at the N-terminal of the Rev peptide. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Ltd. | en |
dc.rights | © 2011 Elsevier Ltd. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Aptamer | en |
dc.subject | Ribonucleopeptide | en |
dc.subject | Biosensor | en |
dc.subject | Modular design | en |
dc.subject | Fluorescence detection | en |
dc.title | Facile conversion of RNA aptamers to modular fluorescent sensors with tunable detection wavelengths. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA11522706 | - |
dc.identifier.jtitle | Bioorganic & medicinal chemistry letters | en |
dc.identifier.volume | 21 | - |
dc.identifier.issue | 15 | - |
dc.identifier.spage | 4503 | - |
dc.identifier.epage | 4506 | - |
dc.relation.doi | 10.1016/j.bmcl.2011.05.120 | - |
dc.textversion | author | - |
dc.identifier.pmid | 21719284 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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