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ファイル | 記述 | サイズ | フォーマット | |
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j.carres.2011.09.031.pdf | 1.62 MB | Adobe PDF | 見る/開く |
タイトル: | Extraction of cellulose-synthesizing activity of Gluconacetobacter xylinus by alkylmaltoside. |
著者: | Hashimoto, Akira Shimono, Kenji Horikawa, Yoshiki Ichikawa, Tsukasa Wada, Masahisa https://orcid.org/0000-0002-3508-3307 (unconfirmed) Imai, Tomoya https://orcid.org/0000-0001-7026-0287 (unconfirmed) Sugiyama, Junji https://orcid.org/0000-0002-5388-4925 (unconfirmed) |
著者名の別形: | 今井, 友也 |
キーワード: | Cellulose synthase CesA/BcsA CesB/BcsB Cellulose I Gluconacetobacter xylinus Membrane protein enzyme |
発行日: | 13-Dec-2011 |
出版者: | Elsevier Ltd. |
誌名: | Carbohydrate research |
巻: | 346 |
号: | 17 |
開始ページ: | 2760 |
終了ページ: | 2768 |
抄録: | This study reinvestigated the synthesis of cellulose in vitro with a well-known cellulose-producing bacterium, Gluconacetobacter xylinus. Alkylmaltoside detergents, which are more frequently used in recent structural biological researches, are uniquely used in this study to solubilize cellulose-synthesizing activity from the cell membrane of G. xylinus. Activity comparable to that previously reported is obtained, while the synthesized cellulose is crystallized into a non-native polymorph of cellulose (cellulose II) as well as the previous studies. In spite of this failure to recover the native activity to synthesize cellulose I microfibril in vitro, the product is a polymer with a degree of polymerization greater than 45 as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). It was thus concluded that the established protocol can solubilize cellulose-synthesizing activity of G. xylinus with polymerizing activity. |
著作権等: | © 2011 Elsevier Ltd. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/151721 |
DOI(出版社版): | 10.1016/j.carres.2011.09.031 |
PubMed ID: | 22070831 |
出現コレクション: | 学術雑誌掲載論文等 |
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