ダウンロード数: 558
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
j.carres.2011.09.031.pdf | 1.62 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Hashimoto, Akira | en |
dc.contributor.author | Shimono, Kenji | en |
dc.contributor.author | Horikawa, Yoshiki | en |
dc.contributor.author | Ichikawa, Tsukasa | en |
dc.contributor.author | Wada, Masahisa | en |
dc.contributor.author | Imai, Tomoya | en |
dc.contributor.author | Sugiyama, Junji | en |
dc.contributor.alternative | 今井, 友也 | ja |
dc.date.accessioned | 2011-12-08T05:04:19Z | - |
dc.date.available | 2011-12-08T05:04:19Z | - |
dc.date.issued | 2011-12-13 | - |
dc.identifier.issn | 0008-6215 | - |
dc.identifier.uri | http://hdl.handle.net/2433/151721 | - |
dc.description.abstract | This study reinvestigated the synthesis of cellulose in vitro with a well-known cellulose-producing bacterium, Gluconacetobacter xylinus. Alkylmaltoside detergents, which are more frequently used in recent structural biological researches, are uniquely used in this study to solubilize cellulose-synthesizing activity from the cell membrane of G. xylinus. Activity comparable to that previously reported is obtained, while the synthesized cellulose is crystallized into a non-native polymorph of cellulose (cellulose II) as well as the previous studies. In spite of this failure to recover the native activity to synthesize cellulose I microfibril in vitro, the product is a polymer with a degree of polymerization greater than 45 as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). It was thus concluded that the established protocol can solubilize cellulose-synthesizing activity of G. xylinus with polymerizing activity. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Elsevier Ltd. | en |
dc.rights | © 2011 Elsevier Ltd. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.subject | Cellulose synthase | en |
dc.subject | CesA/BcsA | en |
dc.subject | CesB/BcsB | en |
dc.subject | Cellulose I | en |
dc.subject | Gluconacetobacter xylinus | en |
dc.subject | Membrane protein enzyme | en |
dc.title | Extraction of cellulose-synthesizing activity of Gluconacetobacter xylinus by alkylmaltoside. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.ncid | AA11522794 | - |
dc.identifier.jtitle | Carbohydrate research | en |
dc.identifier.volume | 346 | - |
dc.identifier.issue | 17 | - |
dc.identifier.spage | 2760 | - |
dc.identifier.epage | 2768 | - |
dc.relation.doi | 10.1016/j.carres.2011.09.031 | - |
dc.textversion | author | - |
dc.identifier.pmid | 22070831 | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。