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dc.contributor.authorHashimoto, Akiraja
dc.contributor.authorShimono, Kenjija
dc.contributor.authorHorikawa, Yoshikija
dc.contributor.authorIchikawa, Tsukasaja
dc.contributor.authorWada, Masahisaja
dc.contributor.authorImai, Tomoyaja
dc.contributor.authorSugiyama, Junjija
dc.contributor.alternative今井, 友也ja
dc.date.accessioned2011-12-08T05:04:19Z-
dc.date.available2011-12-08T05:04:19Z-
dc.date.issued2011-12-13ja
dc.identifier.issn0008-6215ja
dc.identifier.urihttp://hdl.handle.net/2433/151721-
dc.description.abstractThis study reinvestigated the synthesis of cellulose in vitro with a well-known cellulose-producing bacterium, Gluconacetobacter xylinus. Alkylmaltoside detergents, which are more frequently used in recent structural biological researches, are uniquely used in this study to solubilize cellulose-synthesizing activity from the cell membrane of G. xylinus. Activity comparable to that previously reported is obtained, while the synthesized cellulose is crystallized into a non-native polymorph of cellulose (cellulose II) as well as the previous studies. In spite of this failure to recover the native activity to synthesize cellulose I microfibril in vitro, the product is a polymer with a degree of polymerization greater than 45 as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). It was thus concluded that the established protocol can solubilize cellulose-synthesizing activity of G. xylinus with polymerizing activity.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherElsevier Ltd.ja
dc.rights© 2011 Elsevier Ltd.ja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.subjectCellulose synthaseja
dc.subjectCesA/BcsAja
dc.subjectCesB/BcsBja
dc.subjectCellulose Ija
dc.subjectGluconacetobacter xylinusja
dc.subjectMembrane protein enzymeja
dc.titleExtraction of cellulose-synthesizing activity of Gluconacetobacter xylinus by alkylmaltoside.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA11522794ja
dc.identifier.jtitleCarbohydrate researchja
dc.identifier.volume346ja
dc.identifier.issue17ja
dc.identifier.spage2760ja
dc.identifier.epage2768ja
dc.relation.doi10.1016/j.carres.2011.09.031ja
dc.textversionauthorja
dc.identifier.pmid22070831ja
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