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Title: Extraction of cellulose-synthesizing activity of Gluconacetobacter xylinus by alkylmaltoside.
Authors: Hashimoto, Akira
Shimono, Kenji
Horikawa, Yoshiki
Ichikawa, Tsukasa
Wada, Masahisa  kyouindb  KAKEN_id  orcid (unconfirmed)
Imai, Tomoya  kyouindb  KAKEN_id  orcid (unconfirmed)
Sugiyama, Junji  kyouindb  KAKEN_id  orcid (unconfirmed)
Author's alias: 今井, 友也
Keywords: Cellulose synthase
Cellulose I
Gluconacetobacter xylinus
Membrane protein enzyme
Issue Date: 13-Dec-2011
Publisher: Elsevier Ltd.
Journal title: Carbohydrate research
Volume: 346
Issue: 17
Start page: 2760
End page: 2768
Abstract: This study reinvestigated the synthesis of cellulose in vitro with a well-known cellulose-producing bacterium, Gluconacetobacter xylinus. Alkylmaltoside detergents, which are more frequently used in recent structural biological researches, are uniquely used in this study to solubilize cellulose-synthesizing activity from the cell membrane of G. xylinus. Activity comparable to that previously reported is obtained, while the synthesized cellulose is crystallized into a non-native polymorph of cellulose (cellulose II) as well as the previous studies. In spite of this failure to recover the native activity to synthesize cellulose I microfibril in vitro, the product is a polymer with a degree of polymerization greater than 45 as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). It was thus concluded that the established protocol can solubilize cellulose-synthesizing activity of G. xylinus with polymerizing activity.
Rights: © 2011 Elsevier Ltd.
This is not the published version. Please cite only the published version.
DOI(Published Version): 10.1016/j.carres.2011.09.031
PubMed ID: 22070831
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