|Title:||Extraction of cellulose-synthesizing activity of Gluconacetobacter xylinus by alkylmaltoside.|
Wada, Masahisa https://orcid.org/0000-0002-3508-3307 (unconfirmed)
Imai, Tomoya https://orcid.org/0000-0001-7026-0287 (unconfirmed)
Sugiyama, Junji https://orcid.org/0000-0002-5388-4925 (unconfirmed)
|Author's alias:||今井, 友也|
Membrane protein enzyme
|Journal title:||Carbohydrate research|
|Abstract:||This study reinvestigated the synthesis of cellulose in vitro with a well-known cellulose-producing bacterium, Gluconacetobacter xylinus. Alkylmaltoside detergents, which are more frequently used in recent structural biological researches, are uniquely used in this study to solubilize cellulose-synthesizing activity from the cell membrane of G. xylinus. Activity comparable to that previously reported is obtained, while the synthesized cellulose is crystallized into a non-native polymorph of cellulose (cellulose II) as well as the previous studies. In spite of this failure to recover the native activity to synthesize cellulose I microfibril in vitro, the product is a polymer with a degree of polymerization greater than 45 as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). It was thus concluded that the established protocol can solubilize cellulose-synthesizing activity of G. xylinus with polymerizing activity.|
|Rights:||© 2011 Elsevier Ltd.|
This is not the published version. Please cite only the published version.
|Appears in Collections:||Journal Articles|
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