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ファイル | 記述 | サイズ | フォーマット | |
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j.biomaterials.2011.09.050.pdf | 13.18 MB | Adobe PDF | 見る/開く |
タイトル: | Local suppression of pro-inflammatory cytokines and the effects in BMP-2-induced bone regeneration. |
著者: | Ratanavaraporn, Juthamas Furuya, Hiroyuki Tabata, Yasuhiko |
著者名の別形: | 田畑, 泰彦 |
キーワード: | Inflammation suppression Pro-inflammatory cytokines Bone regeneration Triptolide Bone morphogenic protein-2 (BMP-2) Controlled release |
発行日: | Jan-2012 |
出版者: | Elsevier Ltd. |
誌名: | Biomaterials |
巻: | 33 |
号: | 1 |
開始ページ: | 304 |
終了ページ: | 316 |
抄録: | The objective of this study is to investigate the effect of local inflammation suppression on the bone regeneration. Gelatin hydrogels incorporating mixed immunosuppressive triptolide-micelles and bone morphogenic protein-2 (BMP-2) were prepared. The controlled release of both the triptolide and BMP-2 from the hydrogels was observed under in vitro and in vivo conditions. When either J774.1 macrophage-like or MC3T3-E1 osteoblastic cells were cultured in the hydrogels incorporating mixed 2.5, 5 or 10 mg of triptolide-micelles and BMP-2, the expression level of pro- and anti-inflammatory cytokines including interleukin (IL)-6 and IL-10 was down-regulated, but the alkaline phosphatase (ALP) activity was promoted compared with those of hydrogels incorporating BMP-2 without triptolide-micelles. When implanted into a critical-sized bone defect of rats, the hydrogels incorporating mixed 2.5 or 5 mg of triptolide-micelles and BMP-2 showed significantly lower number of neutrophils, lymphocytes, macrophages or dendritic and mast cells infiltrated into the defect, and lower expression level of IL-6, TNF-α, and IL-10 than those incorporating BMP-2 without triptolide-micelles. The reduced local inflammation responses at the defects implanted with the hydrogels incorporating mixed 2.5 or 5 mg of triptolide-micelles and BMP-2 subsequently enhanced the bone regeneration thereat. It is concluded that the proper local modulation of inflammation responses is a promising way to achieve the enhanced bone regeneration. |
著作権等: | © 2011 Elsevier Ltd. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/152300 |
DOI(出版社版): | 10.1016/j.biomaterials.2011.09.050 |
PubMed ID: | 22019155 |
出現コレクション: | 学術雑誌掲載論文等 |
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