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タイトル: | Simple PEG Modification of DNA Aptamer Based on Copper Ion Coordination for Tumor Targeting. |
著者: | Takafuji, Yoshimasa Jo, Jun-Ichiro Tabata, Yasuhiko |
著者名の別形: | 田畑, 泰彦 |
キーワード: | DNA APTAMER POLY(ETHYLENE GLYCOL) METAL COORDINATION TUMOR TARGETING ANTI-TUMOR EFFECT |
発行日: | 2011 |
出版者: | VSP, an imprint of Brill |
誌名: | Journal of biomaterials science. Polymer edition |
巻: | 22 |
号: | 9 |
開始ページ: | 1179 |
終了ページ: | 1198 |
抄録: | A simple modification of a DNA aptamer with poly(ethylene glycol) (PEG) based on metal coordination was developed. N, N-bis(carboxymethyl)-L-lysine (NTA) of a metal chelate residue was chemically introduced to one terminus of PEG. The NTA-introduced PEG (PEG-NTA) chelated Cu(2+) ions form a Cu(2+)-chelated PEG (PEG-Cu). When PEG-Cu was mixed with a DNA aptamer of anti-tumor activity (AS1411) in aqueous solution, a complex of PEG-Cu and AS1411 based on metal coordination was formed. The complex inhibited in vitro tumor growth in a dose-dependent manner. A body distribution study with tumor-bearing mice revealed that PEG-Cu-AS1411 complexes injected intravenously had a significant longer lifetime in the blood circulation and 1.5-2.0-fold higher accumulation in the tumor tissue than free AS1411. Intravenous injection of complexes suppressed the in vivo growth of tumor mass to a significantly greater extent compared with that of free AS1411. The Cu(2+)-coordinated PEG modification is a simple and promising method to enhance accumulation of the aptamer in the tumor, resulting in the augmented anti-tumor effect. |
著作権等: | Copyright 2011 Brill. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/156425 |
PubMed ID: | 20615331 |
関連リンク: | http://www.ingentaconnect.com/content/vsp/bsp/2011/00000022/00000009/art00005 |
出現コレクション: | 学術雑誌掲載論文等 |
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