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タイトル: Isolation, identification, and biological evaluation of Nrf2-ARE activator from the leaves of green perilla (Perilla frutescens var. crispa f. viridis)
著者: Izumi, Yasuhiko  KAKEN_id
Matsumura, Atsuko
Wakita, Seiko
Akagi, Ken-Ichi
Fukuda, Hiroyuki
Kume, Toshiaki  KAKEN_id
Irie, Kazuhiro  KAKEN_id  orcid https://orcid.org/0000-0001-7109-8568 (unconfirmed)
Takada-Takatori, Yuki
Sugimoto, Hachiro
Hashimoto, Tadashi
Akaike, Akinori
著者名の別形: 赤池, 昭紀
キーワード: Chalcone
Green perilla
Nrf2-ARE pathway
Oxidative stress
6-Hydroxydopamine
発行日: 27-Jun-2012
出版者: Elsevier BV
誌名: Free radical biology & medicine
巻: 53
号: 4
開始ページ: 669
終了ページ: 679
抄録: The nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway is a cellular defense system against oxidative stress. Activation of this pathway increases expression of antioxidant enzymes. Epidemiological studies have demonstrated that the consumption of fruits and vegetables is associated with reduced risk of contracting a variety of human diseases. The aim of this study is to find Nrf2-ARE activators in dietary fruits and vegetables. We first attempted to compare the potency of ARE activation in six fruit and six vegetables extracts. Green perilla (Perilla frutescens var. crispa f. viridis) extract exhibited high ARE activity. We isolated the active fraction from green perilla extract through bioactivity-guided fractionation. Based on nuclear magnetic resonance and mass spectrometric analysis, the active ingredient responsible for the ARE activity was identified as 2', 3'-dihydroxy-4', 6'-dimethoxychalcone (DDC). DDC induced the expression of antioxidant enzymes, such as γ-glutamylcysteine synthetase (γ-GCS), NAD(P)H: quinone oxidoreductase-1 (NQO1), and heme oxygenase-1. DDC inhibited the formation of intracellular reactive oxygen species and the cytotoxicity induced by 6-hydroxydopamine. Inhibition of the p38 mitogen-activated protein kinase pathway abolished ARE activation, the induction of γ-GCS and NQO1, and the cytoprotective effect brought about by DDC. Thus, this study demonstrated that DDC contained in green perilla enhanced cellular resistance to oxidative damage through activation of the Nrf2-ARE pathway.
記述: 青ジソから老化やメタボリックシンドローム予防に有望な生体内抗酸化力を高める成分を発見. 京都大学プレスリリース. 2012-08-06.
著作権等: © 2012 Elsevier Inc.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
This is not the published version. Please cite only the published version.
URI: http://hdl.handle.net/2433/159047
DOI(出版社版): 10.1016/j.freeradbiomed.2012.06.021
PubMed ID: 22749808
関連リンク: http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2012/120806_1.htm
出現コレクション:学術雑誌掲載論文等

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