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j.bbrc.2012.05.158.pdf | 301.73 kB | Adobe PDF | 見る/開く |
タイトル: | Stability of acetaldehyde-derived DNA adduct in vitro. |
著者: | Hori, Kimiko Miyamoto, Shin'ichi Yukawa, Yoshiyuki Muto, Manabu https://orcid.org/0000-0002-3127-8203 (unconfirmed) Chiba, Tsutomu Matsuda, Tomonari https://orcid.org/0000-0002-6177-1066 (unconfirmed) |
著者名の別形: | 武藤, 学 |
キーワード: | Alcohol Acetaldehyde DNA adduct N(2)-ethylidene-2′-deoxyguanosine Esophageal cancer Carcinogenesis |
発行日: | 13-Jul-2012 |
出版者: | Elsevier Inc. |
誌名: | Biochemical and biophysical research communications |
巻: | 423 |
号: | 4 |
開始ページ: | 642 |
終了ページ: | 646 |
抄録: | Acetaldehyde (AA) derived from alcoholic beverages is a confirmed carcinogen for esophageal and head and neck cancers. AA forms various DNA adducts and is thought to play a crucial role in carcinogenesis. Transient DNA adducts are usually repaired, but the stability of AA-derived DNA adducts has not been elucidated. We investigated the stability of N(2)-ethylidene-2'-deoxyguanosine (N(2)-ethylidene-dG), a major AA-derived DNA adduct, in cultured cells. First, to determine the optimal concentration of AA for detecting N(2)-ethylidene-dG in cell culture, a dose-response study was performed using HL60 cells of the human promyelocytic leukemia cell line. An AA concentration ≥ 0.01% (1.8 mM) was required to detect N(2)-ethylidene-dG in vitro. We next examined the stability of N(2)-ethylidene-dG. After a 1 or 2h exposure to 0.01% of AA in a tightly sealed bottle, N(2)-ethylidene-dG content was measured by sensitive liquid chromatography tandem mass spectrometry immediately, 24h, and 48 h after exposure. After the 1h exposure, the mean (± SD) N(2)-ethylidene-dG contents were 12.1 ± 1.28, 8.20 ± 0.64, and 6.70 ± 0.52 adducts per 10(7) bases at each postexposure time. After the 2h exposure, N(2)-ethylidene-dG content increased to 21.4 ± 7.50, 10.5 ± 3.61, and 9.83 ± 3.90 adducts per 10(7) bases at each postexposure time. The half-life of this adduct was calculated as ∼35 h in independent experiments. These results indicate that AA exposure from daily alcohol consumption may cause DNA damage and may increase the risk of alcohol-related carcinogenesis. |
著作権等: | © 2012 Elsevier Inc. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/160034 |
DOI(出版社版): | 10.1016/j.bbrc.2012.05.158 |
PubMed ID: | 22683642 |
出現コレクション: | 学術雑誌掲載論文等 |
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