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Title: | Preparation of polymer-based magnetic resonance imaging contrast agent to visualize therapeutic angiogenesis. |
Authors: | Jo, Jun-Ichiro Lin, Xue Nakahara, Teppei Aoki, Ichio Saga, Tsuneo ![]() ![]() ![]() Tabata, Yasuhiko ![]() ![]() |
Author's alias: | 田畑, 泰彦 |
Issue Date: | Dec-2012 |
Publisher: | Mary Ann Liebert, Inc. |
Journal title: | Tissue engineering. Part A |
Volume: | 19 |
Issue: | 1-2 |
Start page: | 30 |
End page: | 39 |
Abstract: | The objective of this study was to design and prepare a new contrast agent of magnetic resonance (MR) imaging for the evaluation of therapeutic angiogenesis. Diethylenetriaminepentaacetic acid (DTPA) residue of a chelator was chemically introduced to dextran with a molecular weight of 74,000 (dextran-DTPA). Cyclic peptide containing an arginine-glycine-aspartic acid (RGD) sequence (cyclic RGD) with an inherent affinity for the α(v)β(3) integrin was then introduced to dextran-DTPA (Cyclic RGD-dextran-DTPA). Gd(3+) was added to cyclic RGD-dextran-DTPA to prepare a dextran-based MR contrast agent (Cyclic RGD-dextran-DTPA-Gd). Cyclic RGD-dextran-DTPA-Gd had affinity for cells expressing the α(v)β(3) integrin and showed a higher longitudinal relaxivity compared with DTPA-Gd of an MR contrast agent clinically used. Right femoral, external iliac, and deep femoral and circumflex arteries and veins were surgically ligated to prepare a mouse model of hindlimb ischemia. A laser Doppler analysis and histological evaluation confirmed that hindlimb ischemia healed naturally and was accompanied by angiogenesis, while α(v)β(3) integrin was expressed in the ischemic-angiogenic region without any treatment. Mice at 7 days after vascular ligation were used as an angiogenesis model. When intravenously injected into mice with hindlimb ischemia, cyclic RGD-dextran-DTPA accumulated in the ischemic-angiogenic region and showed the MR ability to detect the ischemic-angiogenic region. It is concluded that cyclic RGD-dextran-DTPA-Gd is a promising material for evaluation of therapeutic angiogenesis. |
Rights: | This is a copy of an article published in the Tissue Engineering Part A. ©2012 Mary Ann Liebert, Inc. "Tissue Engineering Part A" is available online at: http://online.liebertpub.com. |
URI: | http://hdl.handle.net/2433/168521 |
DOI(Published Version): | 10.1089/ten.TEA.2012.0131 |
PubMed ID: | 22838390 |
Appears in Collections: | Journal Articles |

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