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ten.tea.2012.0131.pdf | 834.4 kB | Adobe PDF | 見る/開く |
タイトル: | Preparation of polymer-based magnetic resonance imaging contrast agent to visualize therapeutic angiogenesis. |
著者: | Jo, Jun-Ichiro Lin, Xue Nakahara, Teppei Aoki, Ichio Saga, Tsuneo https://orcid.org/0000-0001-7801-9316 (unconfirmed) Tabata, Yasuhiko |
著者名の別形: | 田畑, 泰彦 |
発行日: | Dec-2012 |
出版者: | Mary Ann Liebert, Inc. |
誌名: | Tissue engineering. Part A |
巻: | 19 |
号: | 1-2 |
開始ページ: | 30 |
終了ページ: | 39 |
抄録: | The objective of this study was to design and prepare a new contrast agent of magnetic resonance (MR) imaging for the evaluation of therapeutic angiogenesis. Diethylenetriaminepentaacetic acid (DTPA) residue of a chelator was chemically introduced to dextran with a molecular weight of 74,000 (dextran-DTPA). Cyclic peptide containing an arginine-glycine-aspartic acid (RGD) sequence (cyclic RGD) with an inherent affinity for the α(v)β(3) integrin was then introduced to dextran-DTPA (Cyclic RGD-dextran-DTPA). Gd(3+) was added to cyclic RGD-dextran-DTPA to prepare a dextran-based MR contrast agent (Cyclic RGD-dextran-DTPA-Gd). Cyclic RGD-dextran-DTPA-Gd had affinity for cells expressing the α(v)β(3) integrin and showed a higher longitudinal relaxivity compared with DTPA-Gd of an MR contrast agent clinically used. Right femoral, external iliac, and deep femoral and circumflex arteries and veins were surgically ligated to prepare a mouse model of hindlimb ischemia. A laser Doppler analysis and histological evaluation confirmed that hindlimb ischemia healed naturally and was accompanied by angiogenesis, while α(v)β(3) integrin was expressed in the ischemic-angiogenic region without any treatment. Mice at 7 days after vascular ligation were used as an angiogenesis model. When intravenously injected into mice with hindlimb ischemia, cyclic RGD-dextran-DTPA accumulated in the ischemic-angiogenic region and showed the MR ability to detect the ischemic-angiogenic region. It is concluded that cyclic RGD-dextran-DTPA-Gd is a promising material for evaluation of therapeutic angiogenesis. |
著作権等: | This is a copy of an article published in the Tissue Engineering Part A. ©2012 Mary Ann Liebert, Inc. "Tissue Engineering Part A" is available online at: http://online.liebertpub.com. |
URI: | http://hdl.handle.net/2433/168521 |
DOI(出版社版): | 10.1089/ten.TEA.2012.0131 |
PubMed ID: | 22838390 |
出現コレクション: | 学術雑誌掲載論文等 |
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