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Title: Preparation of polymer-based magnetic resonance imaging contrast agent to visualize therapeutic angiogenesis.
Authors: Jo, Jun-Ichiro
Lin, Xue
Nakahara, Teppei
Aoki, Ichio
Saga, Tsuneo  kyouindb  KAKEN_id  orcid (unconfirmed)
Tabata, Yasuhiko  kyouindb  KAKEN_id
Author's alias: 田畑, 泰彦
Issue Date: Dec-2012
Publisher: Mary Ann Liebert, Inc.
Journal title: Tissue engineering. Part A
Volume: 19
Issue: 1-2
Start page: 30
End page: 39
Abstract: The objective of this study was to design and prepare a new contrast agent of magnetic resonance (MR) imaging for the evaluation of therapeutic angiogenesis. Diethylenetriaminepentaacetic acid (DTPA) residue of a chelator was chemically introduced to dextran with a molecular weight of 74,000 (dextran-DTPA). Cyclic peptide containing an arginine-glycine-aspartic acid (RGD) sequence (cyclic RGD) with an inherent affinity for the α(v)β(3) integrin was then introduced to dextran-DTPA (Cyclic RGD-dextran-DTPA). Gd(3+) was added to cyclic RGD-dextran-DTPA to prepare a dextran-based MR contrast agent (Cyclic RGD-dextran-DTPA-Gd). Cyclic RGD-dextran-DTPA-Gd had affinity for cells expressing the α(v)β(3) integrin and showed a higher longitudinal relaxivity compared with DTPA-Gd of an MR contrast agent clinically used. Right femoral, external iliac, and deep femoral and circumflex arteries and veins were surgically ligated to prepare a mouse model of hindlimb ischemia. A laser Doppler analysis and histological evaluation confirmed that hindlimb ischemia healed naturally and was accompanied by angiogenesis, while α(v)β(3) integrin was expressed in the ischemic-angiogenic region without any treatment. Mice at 7 days after vascular ligation were used as an angiogenesis model. When intravenously injected into mice with hindlimb ischemia, cyclic RGD-dextran-DTPA accumulated in the ischemic-angiogenic region and showed the MR ability to detect the ischemic-angiogenic region. It is concluded that cyclic RGD-dextran-DTPA-Gd is a promising material for evaluation of therapeutic angiogenesis.
Rights: This is a copy of an article published in the Tissue Engineering Part A.
©2012 Mary Ann Liebert, Inc.
"Tissue Engineering Part A" is available online at:
DOI(Published Version): 10.1089/ten.TEA.2012.0131
PubMed ID: 22838390
Appears in Collections:Journal Articles

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