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Title: Interleukin-7 receptor controls development and maturation of late stages of thymocyte subpopulations.
Authors: Tani-ichi, Shizue
Shimba, Akihiro
Wagatsuma, Keisuke
Miyachi, Hitoshi
Kitano, Satsuki
Imai, Kumiko
Hara, Takahiro  kyouindb  KAKEN_id
Ikuta, Koichi  kyouindb  KAKEN_id
Author's alias: 谷一, 靖江
Issue Date: 8-Jan-2013
Publisher: National Academy of Sciences
Journal title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 110
Issue: 2
Start page: 612
End page: 617
Abstract: Interleukin (IL)-7 is a cytokine essential for T lymphocyte development and homeostasis. However, little is known about the roles of IL-7 receptor α-chain (IL-7Rα) in late stages of T-cell development. To address this question, we established IL-7Rα-floxed mice and crossed them with CD4-Cre transgenic mice. Resultant IL-7R conditional knockout (IL-7RcKO) mice exhibited marked reduction in CD8 single positive (SP) T cells, regulatory T cells (Tregs), and natural killer T (NKT) cells in thymus. The proportion and proliferation of both mature CD4SP and CD8SP thymocytes were decreased without affecting Runx expression. In addition, expression of the glucocorticoid-induced TNF receptor was reduced in CD4SP and CD8SP thymocytes, and expression of CD5 was decreased in CD8SP thymocytes. IL-7RcKO mice also showed impaired Treg and NKT cell proliferation and inhibition of NKT cell maturation. Bcl-2 expression was reduced in CD4SP and CD8SP thymocytes but not in Tregs and NKT cells, and introduction of a Bcl-2 transgene rescued frequency and CD5 expression of CD8SP thymocytes. Furthermore, IL-7RcKO mice exhibited greatly increased numbers of B cells and, to a lesser extent, γδ T and dendritic cells in thymus. Overall, this study demonstrates that IL-7Rα differentially controls development and maturation of thymocyte subpopulations in late developmental stages and suggests that IL-7R expression on αβ T cells suppresses development of other cell lineages in thymus.
Rights: ©2013 by the National Academy of Sciences
This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/170273
DOI(Published Version): 10.1073/pnas.1219242110
PubMed ID: 23267098
Appears in Collections:Journal Articles

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