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dc.contributor.authorTani-ichi, Shizueja
dc.contributor.authorShimba, Akihiroja
dc.contributor.authorWagatsuma, Keisukeja
dc.contributor.authorMiyachi, Hitoshija
dc.contributor.authorKitano, Satsukija
dc.contributor.authorImai, Kumikoja
dc.contributor.authorHara, Takahiroja
dc.contributor.authorIkuta, Koichija
dc.contributor.alternative谷一, 靖江ja
dc.date.accessioned2013-02-25T01:55:20Z-
dc.date.available2013-02-25T01:55:20Z-
dc.date.issued2013-01-08ja
dc.identifier.issn0027-8424ja
dc.identifier.urihttp://hdl.handle.net/2433/170273-
dc.description.abstractInterleukin (IL)-7 is a cytokine essential for T lymphocyte development and homeostasis. However, little is known about the roles of IL-7 receptor α-chain (IL-7Rα) in late stages of T-cell development. To address this question, we established IL-7Rα-floxed mice and crossed them with CD4-Cre transgenic mice. Resultant IL-7R conditional knockout (IL-7RcKO) mice exhibited marked reduction in CD8 single positive (SP) T cells, regulatory T cells (Tregs), and natural killer T (NKT) cells in thymus. The proportion and proliferation of both mature CD4SP and CD8SP thymocytes were decreased without affecting Runx expression. In addition, expression of the glucocorticoid-induced TNF receptor was reduced in CD4SP and CD8SP thymocytes, and expression of CD5 was decreased in CD8SP thymocytes. IL-7RcKO mice also showed impaired Treg and NKT cell proliferation and inhibition of NKT cell maturation. Bcl-2 expression was reduced in CD4SP and CD8SP thymocytes but not in Tregs and NKT cells, and introduction of a Bcl-2 transgene rescued frequency and CD5 expression of CD8SP thymocytes. Furthermore, IL-7RcKO mice exhibited greatly increased numbers of B cells and, to a lesser extent, γδ T and dendritic cells in thymus. Overall, this study demonstrates that IL-7Rα differentially controls development and maturation of thymocyte subpopulations in late developmental stages and suggests that IL-7R expression on αβ T cells suppresses development of other cell lineages in thymus.ja
dc.format.mimetypeapplication/pdfja
dc.language.isoengja
dc.publisherNational Academy of Sciencesja
dc.rights©2013 by the National Academy of Sciencesja
dc.rightsThis is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。ja
dc.titleInterleukin-7 receptor controls development and maturation of late stages of thymocyte subpopulations.ja
dc.type.niitypeJournal Articleja
dc.identifier.ncidAA00786025ja
dc.identifier.jtitleProceedings of the National Academy of Sciences of the United States of Americaja
dc.identifier.volume110ja
dc.identifier.issue2ja
dc.identifier.spage612ja
dc.identifier.epage617ja
dc.relation.doi10.1073/pnas.1219242110ja
dc.textversionauthorja
dc.identifier.pmid23267098ja
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