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タイトル: | Interleukin-7 receptor controls development and maturation of late stages of thymocyte subpopulations |
著者: | Tani-ichi, Shizue Shimba, Akihiro Wagatsuma, Keisuke Miyachi, Hitoshi Kitano, Satsuki Imai, Kumiko Hara, Takahiro https://orcid.org/0000-0002-3845-8434 (unconfirmed) Ikuta, Koichi https://orcid.org/0000-0003-1319-1021 (unconfirmed) |
著者名の別形: | 谷一, 靖江 |
発行日: | 8-Jan-2013 |
出版者: | National Academy of Sciences |
誌名: | Proceedings of the National Academy of Sciences of the United States of America |
巻: | 110 |
号: | 2 |
開始ページ: | 612 |
終了ページ: | 617 |
抄録: | Interleukin (IL)-7 is a cytokine essential for T lymphocyte development and homeostasis. However, little is known about the roles of IL-7 receptor α-chain (IL-7Rα) in late stages of T-cell development. To address this question, we established IL-7Rα-floxed mice and crossed them with CD4-Cre transgenic mice. Resultant IL-7R conditional knockout (IL-7RcKO) mice exhibited marked reduction in CD8 single positive (SP) T cells, regulatory T cells (Tregs), and natural killer T (NKT) cells in thymus. The proportion and proliferation of both mature CD4SP and CD8SP thymocytes were decreased without affecting Runx expression. In addition, expression of the glucocorticoid-induced TNF receptor was reduced in CD4SP and CD8SP thymocytes, and expression of CD5 was decreased in CD8SP thymocytes. IL-7RcKO mice also showed impaired Treg and NKT cell proliferation and inhibition of NKT cell maturation. Bcl-2 expression was reduced in CD4SP and CD8SP thymocytes but not in Tregs and NKT cells, and introduction of a Bcl-2 transgene rescued frequency and CD5 expression of CD8SP thymocytes. Furthermore, IL-7RcKO mice exhibited greatly increased numbers of B cells and, to a lesser extent, γδ T and dendritic cells in thymus. Overall, this study demonstrates that IL-7Rα differentially controls development and maturation of thymocyte subpopulations in late developmental stages and suggests that IL-7R expression on αβ T cells suppresses development of other cell lineages in thymus. |
著作権等: | ©2013 by the National Academy of Sciences この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/170273 |
DOI(出版社版): | 10.1073/pnas.1219242110 |
PubMed ID: | 23267098 |
出現コレクション: | 学術雑誌掲載論文等 |
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