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タイトル: Diet-induced changes in Ucp1 expression in bovine adipose tissues.
著者: Asano, Hiroki
Yamada, Tomoya
Hashimoto, Osamu
Umemoto, Takenao
Sato, Ryo
Ohwatari, Shiori
Kanamori, Yohei
Terachi, Tomohiro
Funaba, Masayuki  kyouindb  KAKEN_id  orcid https://orcid.org/0000-0002-4303-6682 (unconfirmed)
Matsui, Tohru  KAKEN_id  orcid https://orcid.org/0000-0003-1011-7053 (unconfirmed)
著者名の別形: 舟場, 正幸
キーワード: Brown adipocytes
Ucp1
White adipose tissues
Mature cattle
発行日: Apr-2013
出版者: Elsevier Inc.
誌名: General and comparative endocrinology
巻: 184
開始ページ: 87
終了ページ: 92
抄録: Brown adipocytes, which regulate non-shivering thermogenesis, have been believed to exist in a limited number of mammalian species, and only under limited physiological conditions. Recent discoveries indicate that adult humans possess a significant number of functional brown adipocytes. This study explores the regulatory emergence of brown adipocytes in white adipose tissue (WAT) depots of fattening cattle. RT-PCR analyses indicated significant expression of Ucp1, a brown adipocyte-specific gene, in the WAT of 31-month-old Japanese Black steers. Immunohistochemical analysis revealed that Ucp1-positive small adipocytes were dispersed in the subcutaneous WAT. Next, we examined expression level of Ucp1 and other brown adipocyte-selective genes such as Pgc1α, Cidea, Dio2, Cox1, Cox7a1 and Cox8b in WAT of 30-month-old steers fed either diet with low protein/energy content (roughage diet) or that with high protein/energy content (concentrate diet) for 20months. Ucp1 expression in the subcutaneous WAT was significantly higher in the concentrate diet group than in the roughage diet group. Furthermore, the higher Ucp1 expression levels were limited to the subcutaneous WAT, and no differences between groups were detected in the mesenteric, perirenal, intermuscular or intramuscular WAT. Expression of Dio2, Cox1 and Cox8b was higher in the subcutaneous WAT but not in the mesenteric WAT of the concentrate diet group. Furthermore, expression of Prdm16, a positive regulator of differentiation toward brown adipocyte-lineage cells, and expression of leptin, a molecule that enhances activity of brown adipocytes, were significantly higher in the subcutaneous WAT of the concentrate diet group. This study demonstrates the presence of brown adipocytes in WAT depots of fattening cattle, and suggests the diet-related modulation of expression of genes predominantly expressed in brown adipocytes.
著作権等: © 2013 Elsevier Inc.
This is not the published version. Please cite only the published version.
この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。
URI: http://hdl.handle.net/2433/171241
DOI(出版社版): 10.1016/j.ygcen.2013.01.006
PubMed ID: 23370305
出現コレクション:学術雑誌掲載論文等

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