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タイトル: Anti-prion activity of an RNA aptamer and its structural basis.
著者: Mashima, Tsukasa  kyouindb  KAKEN_id
Nishikawa, Fumiko
Kamatari, Yuji O
Fujiwara, Hiromichi
Saimura, Masayuki
Nagata, Takashi  kyouindb  KAKEN_id
Kodaki, Tsutomu  kyouindb  KAKEN_id
Nishikawa, Satoshi
Kuwata, Kazuo
Katahira, Masato  kyouindb  KAKEN_id
著者名の別形: 片平, 正人
発行日: Jan-2013
出版者: Oxford University Press
誌名: Nucleic acids research
巻: 41
号: 2
開始ページ: 1355
終了ページ: 1362
抄録: Prion proteins (PrPs) cause prion diseases, such as bovine spongiform encephalopathy. The conversion of a normal cellular form (PrP(C)) of PrP into an abnormal form (PrP(Sc)) is thought to be associated with the pathogenesis. An RNA aptamer that tightly binds to and stabilizes PrP(C) is expected to block this conversion and to thereby prevent prion diseases. Here, we show that an RNA aptamer comprising only 12 residues, r(GGAGGAGGAGGA) (R12), reduces the PrP(Sc) level in mouse neuronal cells persistently infected with the transmissible spongiform encephalopathy agent. Nuclear magnetic resonance analysis revealed that R12, folded into a unique quadruplex structure, forms a dimer and that each monomer simultaneously binds to two portions of the N-terminal half of PrP(C), resulting in tight binding. Electrostatic and stacking interactions contribute to the affinity of each portion. Our results demonstrate the therapeutic potential of an RNA aptamer as to prion diseases.
著作権等: © The Author(s) 2012. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.
URI: http://hdl.handle.net/2433/173326
DOI(出版社版): 10.1093/nar/gks1132
PubMed ID: 23180780
出現コレクション:学術雑誌掲載論文等

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