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dc.contributor.author | Cui, Guangwei | en |
dc.contributor.author | Hara, Takahiro | en |
dc.contributor.author | Simmons, Szandor | en |
dc.contributor.author | Wagatsuma, Keisuke | en |
dc.contributor.author | Abe, Akifumi | en |
dc.contributor.author | Miyachi, Hitoshi | en |
dc.contributor.author | Kitano, Satsuki | en |
dc.contributor.author | Ishii, Masaru | en |
dc.contributor.author | Tani-Ichi, Shizue | en |
dc.contributor.author | Ikuta, Koichi | en |
dc.contributor.alternative | 生田, 宏一 | ja |
dc.date.accessioned | 2014-02-04T08:00:27Z | - |
dc.date.available | 2014-02-04T08:00:27Z | - |
dc.date.issued | 2014-01-21 | - |
dc.identifier.issn | 1091-6490 | - |
dc.identifier.uri | http://hdl.handle.net/2433/180642 | - |
dc.description | サイトカインIL-15を産生する細胞の可視化に成功 -免疫系の微小環境の解明に期待-. 京都大学プレスリリース. 2014-01-21. | ja |
dc.description.abstract | IL-15 is a cytokine critical for development, maintenance, and response of T cells, natural killer (NK) cells, NK T cells, and dendritic cells. However, the identity and distribution of IL-15-expressing cells in lymphoid organs are not well understood. To address these questions, we established and analyzed IL-15-CFP knock-in mice. We found that IL-15 was highly expressed in thymic medulla, and medullary thymic epithelial cells with high MHC class II expression were the major source of IL-15. In bone marrow, IL-15 was detected primarily in VCAM-1(+)PDGFRβ(+)CD31(-)Sca-1(-) stromal cells, which corresponded to previously described CXCL12-abundant reticular cells. In lymph nodes, IL-15-expressing cells were mainly distributed in the T-cell zone and medulla. IL-15 was expressed in some fibroblastic reticular cells and gp38(-)CD31(-) double-negative stromal cells in the T-cell zone. Blood endothelial cells, including all high endothelial venules, also expressed high IL-15 levels in lymph nodes, whereas lymphatic endothelial cells (LECs) lacked IL-15 expression. In spleen, IL-15 was expressed in VCAM-1(+) stromal cells, where its expression increased as mice aged. Finally, IL-15 expression in blood and LECs of peripheral lymphoid organs significantly increased in LPS-induced inflammation. Overall, we have identified and characterized several IL-15-expressing cells in primary and secondary lymphoid organs, providing a unique perspective of IL-15 niche in immune microenvironment. This study also suggests that some stromal cells express IL-7 and IL-15 differentially and suggests a way to functionally classify different stromal cell subsets. | en |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | National Academy of Sciences | en |
dc.rights | © 2014 National Academy of Sciences. | en |
dc.rights | This is not the published version. Please cite only the published version. | en |
dc.rights | この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | ja |
dc.title | Characterization of the IL-15 niche in primary and secondary lymphoid organs in vivo. | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Proceedings of the National Academy of Sciences of the United States of America | en |
dc.identifier.volume | 111 | - |
dc.identifier.issue | 5 | - |
dc.identifier.spage | 1915 | - |
dc.identifier.epage | 1920 | - |
dc.relation.doi | 10.1073/pnas.1318281111 | - |
dc.textversion | author | - |
dc.identifier.pmid | 24449915 | - |
dc.relation.url | http://www.kyoto-u.ac.jp/ja/news_data/h/h1/news6/2013_1/140121_1.htm | - |
dcterms.accessRights | open access | - |
出現コレクション: | 学術雑誌掲載論文等 |
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