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j.jchromb.2014.01.052.pdf | 557.92 kB | Adobe PDF | 見る/開く |
タイトル: | Utilization of a precolumn with size exclusion and reversed-phase modes for size-exclusion chromatographic analysis of polysorbate-containing protein aggregates. |
著者: | Fukuda, Jun Iura, Takafumi Yanagihara, Shigehiro Kano, Kenji |
著者名の別形: | 加納, 健司 |
キーワード: | Size-exclusion chromatography Polysorbate Protein aggregation MSpak GF-4A Temperature-dependent retention |
発行日: | 15-Mar-2014 |
出版者: | Elsevier B.V. |
誌名: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
巻: | 953-954 |
開始ページ: | 68 |
終了ページ: | 72 |
抄録: | Size-exclusion chromatography (SEC) is a useful method for quantification of protein aggregates because of its high throughput capacity and highly quantitative performance. One of the problems in this method concerns polysorbates, which are well-known additives for protein-containing products to prevent protein aggregation, but frequently interfere with the photometric detection of protein aggregates. We developed a new SEC method that can separate polysorbates from protein sample solutions in an on-line mode with a precolumn with size exclusion and reversed-phase mixed modes. The precolumn can effectively trap polysorbates in aqueous mobile phase, and the trapped polysorbates are easily eluted with acetonitrile-containing aqueous mobile phase to clean the precolumn. Small parts of protein aggregates may be also trapped on the precolumn depending on temperature and proteins. Setting appropriate column temperature can minimize such inconvenient trapping of aggregates. |
著作権等: | © 2014 Elsevier B.V. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/185681 |
DOI(出版社版): | 10.1016/j.jchromb.2014.01.052 |
PubMed ID: | 24576768 |
出現コレクション: | 学術雑誌掲載論文等 |
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